MUC20 Monoclonal antibody

MUC20 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG3

Reactivity

human, canine

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1G9B2

Cat no : 68714-1-Ig

Synonyms

UNQ2782/PRO7170, Mucin-20, MUC-20, MUC 20, KIAA1359

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Validation Data Gallery

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  WB analysis using 68714-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68714-1-Ig (MUC20 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 68714-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using MUC20 antibody (68714-1-Ig, Clone: 1G9B2 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

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Tested Applications

• Positive WB detected in: MDCK cells, HEK-293 cells, HK-2 cells, A549 cells, SK-BR-3 cells
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68714-1-Ig targets MUC20 in WB, IF/ICC, ELISA applications and shows reactivity with human, canine samples.

• Tested Reactivity: human, canine
• Host / Isotype: Mouse / IgG3
• Class: Monoclonal
• Type: Antibody
• Immunogen: MUC20 fusion protein Ag31840
• Full Name: mucin 20, cell surface associated
• Calculated Molecular Weight: 55 kDa, 71 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC029267
• Gene Symbol: MUC20
• Gene ID (NCBI): 200958
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MUC20 contains serine, threonine, and proline-rich repeats in its extracellular domain and is likely to be extensively O-glycosylated. MUC20 blocks GRB2 recruitment to MET thus suppressing the GRB2-RAS pathway and inhibiting HGF-induced proliferation of MMP1 and MMP9 expression (PMID: 15314156). MUC20 is highly expressed in the kidney and localized in the proximal tubules but not in the glomerulus or distal tubules(PMID: 14565953). MUC20 was detected in most of the male urogenital tract epithelia, except for epididymis(PMID: 16997931). The expression of MUC20 is up-regulated in the kidneys of patients with immunoglobulin A nephropathy, in an animal model of lupus nephritis, and in mice with acute renal injury caused by cisplatin administration or unilateral ureteral obstruction(PMID: 14565953).