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MUC20 Monoclonal antibody

MUC20 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG3

Reactivity

human, canine

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1G9B2

Cat no : 68714-1-Ig

Synonyms

UNQ2782/PRO7170, Mucin-20, MUC-20, MUC 20, KIAA1359

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MUC20 Monoclonal antibody (68714-1-Ig)

    WB analysis using 68714-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68714-1-Ig (MUC20 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using MUC20 Monoclonal antibody (68714-1-Ig)

    IF Staining of HepG2 using 68714-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using MUC20 antibody (68714-1-Ig, Clone: 1G9B2 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

Tested Applications

Positive WB detected inMDCK cells, HEK-293 cells, HK-2 cells, A549 cells, SK-BR-3 cells
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68714-1-Ig targets MUC20 in WB, IF/ICC, ELISA applications and shows reactivity with human, canine samples.

Tested Reactivity human, canine
Host / Isotype Mouse / IgG3
Class Monoclonal
Type Antibody
Immunogen MUC20 fusion protein Ag31840
Full Name mucin 20, cell surface associated
Calculated Molecular Weight 55 kDa, 71 kDa
Observed Molecular Weight 70 kDa
GenBank Accession NumberBC029267
Gene Symbol MUC20
Gene ID (NCBI) 200958
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MUC20 contains serine, threonine, and proline-rich repeats in its extracellular domain and is likely to be extensively O-glycosylated. MUC20 blocks GRB2 recruitment to MET thus suppressing the GRB2-RAS pathway and inhibiting HGF-induced proliferation of MMP1 and MMP9 expression (PMID: 15314156). MUC20 is highly expressed in the kidney and localized in the proximal tubules but not in the glomerulus or distal tubules(PMID: 14565953). MUC20 was detected in most of the male urogenital tract epithelia, except for epididymis(PMID: 16997931). The expression of MUC20 is up-regulated in the kidneys of patients with immunoglobulin A nephropathy, in an animal model of lupus nephritis, and in mice with acute renal injury caused by cisplatin administration or unilateral ureteral obstruction(PMID: 14565953).

Protocols

Product Specific Protocols
WB protocol for MUC20 antibody 68714-1-IgDownload protocol
IF protocol for MUC20 antibody 68714-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols