Cytokeratin 1-specific Recombinant antibody

• 

  WB analysis using 82972-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82972-1-RR (KRT1-specific antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

• 

  FC experiment of A431 using 82972-1-RR

  1x10^6 A431 cells were intracellularly stained with 0.25 ug Anti-Human KRT1-specific (82972-1-RR, Clone:230261F9) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP)(blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Cytokeratin 1-specific Uni-rAb^TM Recombinant Antibody for WB, FC (Intra), ELISA

Cat No. 82972-1-RR

Clone No.230261F9

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, FC (Intra), ELISA

Synonyms

Cytokeratin 1, Cytokeratin-1, Cytokeratin1, Cytokeratin,Keratin, CK-1

---

Tested Applications

• Positive WB detected in: mouse skin tissue, rat skin tissue
• Positive FC (Intra) detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

82972-1-RR targets Cytokeratin 1-specific in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: keratin 1
• Calculated Molecular Weight: 66 kDa
• Observed Molecular Weight: 67 kDa
• GenBank Accession Number: NM_006121
• Gene Symbol: KRT1
• Gene ID (NCBI): 3848
• RRID: AB_3670720
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P04264
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin-1 is type II keratin. It is a specific marker for terminal differentiation in mammalian epidermis. It is specifically expressed in the spinous and granular layers of the epidermis with family member KRT10 and mutations in these genes have been associated with bullous congenital ichthyosiform erythroderma.