CoraLite® Plus 488-conjugated Cytokeratin 1-specific Recombinant antibody

KRT1-specific Recombinant Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

230261F9

Cat no : CL488-82972

Synonyms

67 kDa cytokeratin, CK 1, CK1, Cytokeratin 1, EHK1, Hair alpha protein, K1, keratin 1, KRT1, KRT1 absorb, KRT1A, KRTA, Type II keratin Kb1

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Validation Data Gallery

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  FC experiment of MCF-7 using CL488-82972

  1x10^6 MCF-7 cells were intracellularly stained with 0.8 ug Coralite® Plus 488 Krt1-Specific Recombinant Antibody (CL488-82972, Clone:230261F9)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  FC experiment of A431 using CL488-82972

  1x10^6 A431 cells were intracellularly stained with 0.8 ug Coralite® Plus 488 Krt1-Specific Recombinant Antibody (CL488-82972, Clone:230261F9)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive FC (Intra) detected in: A431 cells, MCF-7 cells
• Positive FC detected in: A431 cells, MCF-7 cells

Recommended dilution

• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
• Flow Cytometry (FC): FC : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-82972 targets KRT1-specific in FC (Intra) applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: keratin 1
• Calculated Molecular Weight: 66 kDa
• Observed Molecular Weight: 67 kDa
• GenBank Accession Number: NM_006121
• Gene Symbol: KRT1
• Gene ID (NCBI): 3848
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin-1 is type II keratin. It is a specific marker for terminal differentiation in mammalian epidermis. It is specifically expressed in the spinous and granular layers of the epidermis with family member KRT10 and mutations in these genes have been associated with bullous congenital ichthyosiform erythroderma.