Cytokeratin 1-specific Recombinant antibody, PBS Only

Cytokeratin 1-specific Uni-rAb^TM Recombinant Antibody for WB, FC (Intra), Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

230261F9

Cat no : 82972-1-PBS

Synonyms

KRT1-specific, Cytokeratin 1, Cytokeratin-1, Cytokeratin1, Cytokeratin,Keratin

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Validation Data Gallery

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  WB analysis using 82972-1-RR (same clone as 82972-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82972-1-RR (KRT1-specific antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82972-1-PBS in a different storage buffer formulation.

• 

  FC experiment of A431 using 82972-1-RR (same clone as 82972-1-PBS)

  1x10^6 A431 cells were intracellularly stained with 0.25 ug Anti-Human KRT1-specific (82972-1-RR, Clone:230261F9) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP)(blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82972-1-PBS in a different storage buffer formulation.

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Product Information

82972-1-PBS targets Cytokeratin 1-specific in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: keratin 1
• Calculated Molecular Weight: 66 kDa
• Observed Molecular Weight: 67 kDa
• GenBank Accession Number: NM_006121
• Gene Symbol: KRT1
• Gene ID (NCBI): 3848
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin-1 is type II keratin. It is a specific marker for terminal differentiation in mammalian epidermis. It is specifically expressed in the spinous and granular layers of the epidermis with family member KRT10 and mutations in these genes have been associated with bullous congenital ichthyosiform erythroderma.