CoraLite®594-conjugated KLK11 Monoclonal antibody

KLK11 Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

Human, Pig, Rat

Applications

FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1E2B4

Cat no : CL594-67427

Synonyms

Kallikrein 11, KLK11, PRSS20, Trypsin-like protease, UNQ649/PRO1279, Kallikrein11, Kallikrein-11, Kallikrein-11 inactive chain 1, Kallikrein-11 inactive chain 2, KLK 11, 1E2B4, EC:3.4.21.-

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Validation Data Gallery

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  FC experiment of HepG2 using CL594-67427

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human KLK11 (CL594-67427, Clone:1E2B4) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

• Positive FC (Intra) detected in: HepG2 cells
• Positive FC detected in: HepG2 cells

Recommended dilution

• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-67427 targets KLK11 in FC (Intra) applications and shows reactivity with Human, Pig, Rat samples.

• Tested Reactivity: Human, Pig, Rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: KLK11 fusion protein Ag29895
• Full Name: kallikrein-related peptidase 11
• Calculated Molecular Weight: 250 aa, 27 kDa
• Observed Molecular Weight: 30-40 kDa
• GenBank Accession Number: BC022068
• Gene Symbol: KLK11
• Gene ID (NCBI): 11012
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

KLK11(Kallikrein-11) is also named as PRSS20, TLSP and belongs to the peptidase S1 family and Kallikrein subfamily. It is a multifunctinal protease cleaving several substrates for kallikrein and trypsin and involved in normal physiology processes in bronchus. This full length protein has a signal peptide, a propeptide and four glycosylation sites. It has 4 isoforms produced by alternative splicing with the molecular weight of 31 kDa, 27 kDa,34 kDa and 30 kDa.