KLK11 Monoclonal antibody

KLK11 Monoclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

Publications

2

CloneNo.

1E2B4

Cat no : 67427-1-Ig

Synonyms

Kallikrein 11, Kallikrein-11 inactive chain 1, Kallikrein-11, Kallikrein11, EC:3.4.21.-

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Validation Data Gallery

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  WB analysis using 67427-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67427-1-Ig (KLK11 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis using 67427-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67427-1-Ig (KLK11 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 67427-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67427-1-Ig (KLK11 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 67427-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67427-1-Ig (KLK11 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 67427-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67427-1-Ig (KLK11 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 67427-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67427-1-Ig (KLK11 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 67427-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67427-1-Ig (KLK11 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human breast cancer using 67427-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using KLK11 antibody (67427-1-Ig, Clone: 1E2B4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of human breast cancer using 67427-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using KLK11 antibody (67427-1-Ig, Clone: 1E2B4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: TT cells, DU 145 cells, pig brain tissue, LNCaP cells, PC-3 cells, Jurkat cells, rat brain tissue, A431 cells, HaCaT cells, HT-29 cells, SW 1990 cells, mouse brain tissue, rat skin tissue
• Positive IHC detected in: human breast cancer tissue, human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: human breast cancer tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IHC: See 1 publications below

Product Information

67427-1-Ig targets KLK11 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: KLK11 fusion protein Ag29895
• Full Name: kallikrein-related peptidase 11
• Calculated Molecular Weight: 250 aa, 27 kDa
• Observed Molecular Weight: 30-40 kDa
• GenBank Accession Number: BC022068
• Gene Symbol: KLK11
• Gene ID (NCBI): 11012
• RRID: AB_2882666
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

KLK11(Kallikrein-11) is also named as PRSS20, TLSP and belongs to the peptidase S1 family and Kallikrein subfamily. It is a multifunctinal protease cleaving several substrates for kallikrein and trypsin and involved in normal physiology processes in bronchus. This full length protein has a signal peptide, a propeptide and four glycosylation sites. It has 4 isoforms produced by alternative splicing with the molecular weight of 31 kDa, 27 kDa,34 kDa and 30 kDa.

Publications

Species	Application	Title
human	WB	J Bioenerg Biomembr; KLK11 acts as a tumor-inhibitor in laryngeal squamous cell carcinoma through the inactivation of Akt/Wnt/β-catenin signaling.; Authors - Ruimin Zhao
human	WB,IHC	Carcinogenesis; miR-1304 targets KLK11 to regulate gastric cancer cell proliferation through the mTOR signaling pathway; Authors - Yi Ding