CoraLite® Plus 488-conjugated KLF5 Polyclonal antibody

• Featured Product
• KD/KO Validated

KLF5 Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-21017

Synonyms

GC-box-binding protein 2, Colon krueppel-like factor, CKLF, BTE-binding protein 2, BTEB2

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Validation Data Gallery

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  IF Staining of HeLa using CL488-21017

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 KLF5 antibody (CL488-21017) at dilution of 1:200, CL594-Phalloidin (red).

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  FC experiment of A549 using CL488-21017

  1x10^6 A549 cells were intracellularly stained with 0.8 ug Coralite® Plus 488 Klf5 Polyclonal Antibody (CL488-21017)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

• Positive IF/ICC detected in: HeLa cells
• Positive FC (Intra) detected in: A549 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-21017 targets KLF5 in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: KLF5 fusion protein Ag15267
• Full Name: Kruppel-like factor 5 (intestinal)
• Calculated Molecular Weight: 457 aa, 51 kDa
• Observed Molecular Weight: 51-55 kDa
• GenBank Accession Number: BC042131
• Gene Symbol: KLF5
• Gene ID (NCBI): 688
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

KLF5, also known as BTEB2 and IKLF, is a member of the Krueppel-like transcription factor family. It is a transcription factor for SMemb/non-muscle myosin heavy chain-B (NMHC-B). It is a molecular marker of phenotypically modulated smooth-muscle cells. KLF5 seems to be a key element linking external stress and cardiovascular remodeling. This antibody detects the 50-60 kDa isoform 1 of KLF5. The antibody can recognize isoform 2 (33 kDa) and isoform 3 (18 kDa) too. 85 kDa, 95 kDa and 120 kDa of SUMOylated KLF5 were detected (PMID: 18500350).