FC experiment of human whole blood cells using CL647-65243
1X10^6 human whole blood cells were surface stained with 0.2 ug CoraLite® Plus 647 Anti-Mouse IgM Isotype Control (red), CoraLite 650 Plus Anti-Human STRO-1 (green), or unstained (blue). Cells were not fixed. CD45dim, CD34+ lymphocytes were gated.
1X10^6 human whole blood cells were surface stained with 0.2 ug CoraLite® Plus 647 Anti-Mouse IgM Isotype Control (red), CoraLite 650 Plus Anti-Human STRO-1 (green), or unstained (blue). Cells were not fixed. CD45dim, CD34+ lymphocytes were gated.
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
Product Information
CL647-65243 targets IgM Isotype Control in FC applications and shows reactivity with n/a samples.
Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.
Background Information
The MOPC-104E immunoglobulin is useful as an isotype-matched control for mouse IgM antibodies. B1355S dextran, nigerosyl-alpha (1-3)-nigerose, and nigerosyl-alpha (1-3)-glucose have abilities to bind to MOPC-104E immunoglobulin (PMID: 6165777). This antibody has been quality-tested for flow cytometry as negative control.
Protocols
Product Specific Protocols
FC protocol for CL Plus 647 IgM Isotype Control antibody CL647-65243
FC experiment of human whole blood cells using CL647-65243
1X10^6 human whole blood cells were surface stained with 0.2 ug CoraLite® Plus 647 Anti-Mouse IgM Isotype Control (red), CoraLite 650 Plus Anti-Human STRO-1 (green), or unstained (blue). Cells were not fixed. CD45dim, CD34+ lymphocytes were gated.
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
IgM Isotype Control Monoclonal antibody
Catalog Number
CL647-65243
Citations
-
Dilutions
Applications
FC
Reactivity
n/a
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
