CoraLite® Plus 555 Mouse IgM Isotype Control (MOPC-104E)

IgM Isotype Control Monoclonal Antibody for FC

Host / Isotype

Mouse / IgM, lambda

Reactivity

n/a

Applications

FC

Conjugate

CoraLite® Plus 555 Fluorescent Dye

CloneNo.

MOPC-104E

Cat no : CL555-65243

Synonyms

Mouse IgM Isotype Control, IgM

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Validation Data Gallery

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  FC experiment of human peripheral blood platelets using CL555-65243

  1X10^6 human peripheral blood platelets were surface stained with 0.2 ug CoraLite® Plus 555 Anti-Mouse IgM Isotype Control (CL555-65243, Clone:MOPC-104E) (red), unstained (blue), or CoraLite® Plus 555 Anti-Human CD36 (SMΦ) (green). Cells were not fixed.

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Tested Applications

Positive FC detected in

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL555-65243 targets IgM Isotype Control in FC applications and shows reactivity with n/a samples.

• Tested Reactivity: n/a
• Host / Isotype: Mouse / IgM, lambda
• Class: Monoclonal
• Type: Antibody
• Immunogen: N/A
• Full Name: IgM Isotype Control
• RRID: AB_3084609
• Conjugate: CoraLite® Plus 555 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 554 nm / 570 nm
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

The MOPC-104E immunoglobulin is useful as an isotype-matched control for mouse IgM antibodies. B1355S dextran, nigerosyl-alpha (1-3)-nigerose, and nigerosyl-alpha (1-3)-glucose have abilities to bind to MOPC-104E immunoglobulin (PMID: 6165777). This antibody has been quality-tested for flow cytometry as negative control.