FC experiment of human whole blood using 65243-1-Ig
Human whole blood was treated with ACK lysis buffer then 1x10^6 cells were surface stained with 0.2 ug Mouse IgM Isotype Control (MOPC-104E) (65243-1-Ig) and CoraLite®647-conjugated AffiniPure F(ab')2 Fragment Goat Anti-Mouse IgG (H+L) (SA00014-8) (red), or CoraLite®647-conjugated AffiniPure F(ab')2 Fragment Goat Anti-Mouse IgG (H+L) (SA00014-8) alone (black dashed), or unstained (blue). Cells were not fixed. Lymphocytes were gated.
Human whole blood was treated with ACK lysis buffer then 1x10^6 cells were surface stained with 0.2 ug Mouse IgM Isotype Control (MOPC-104E) (65243-1-Ig) and CoraLite®647-conjugated AffiniPure F(ab')2 Fragment Goat Anti-Mouse IgG (H+L) (SA00014-8) (red), or CoraLite®647-conjugated AffiniPure F(ab')2 Fragment Goat Anti-Mouse IgG (H+L) (SA00014-8) alone (black dashed), or unstained (blue). Cells were not fixed. Lymphocytes were gated.
FC experiment of human peripheral blood platelets using 65243-1-Ig
100ul human platelet-rich plasma (diluted 1:2 in PBS) was surface stained with 0.2 ug Mouse IgM Isotype Control (65243-1-Ig, Clone: MOPC-104E) (red) or Anti-Human CD36 (65248-1-Ig) (green) or unstained (blue) and CoraLite®647-conjugated F(ab')2 Fragment Goat Anti-Mouse IgG (H+L) (SA00014-8). Cells were not fixed.
100ul human platelet-rich plasma (diluted 1:2 in PBS) was surface stained with 0.2 ug Mouse IgM Isotype Control (65243-1-Ig, Clone: MOPC-104E) (red) or Anti-Human CD36 (65248-1-Ig) (green) or unstained (blue) and CoraLite®647-conjugated F(ab')2 Fragment Goat Anti-Mouse IgG (H+L) (SA00014-8). Cells were not fixed.
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This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
Product Information
65243-1-Ig targets IgM Isotype Control in FC applications and shows reactivity with n/a samples.
Store at 2-8°C. Stable for one year after shipment.
Background Information
The MOPC-104E immunoglobulin is useful as an isotype-matched control for mouse IgM antibodies. B1355S dextran, nigerosyl-alpha (1-3)-nigerose, and nigerosyl-alpha (1-3)-glucose have abilities to bind to MOPC 104E immunoglobulin (PMID: 6165777). This antibody has been quality-tested for flow cytometry as negative control.
Protocols
Product Specific Protocols
FC protocol for IgM Isotype Control antibody 65243-1-Ig
FC experiment of human whole blood using 65243-1-Ig
Human whole blood was treated with ACK lysis buffer then 1x10^6 cells were surface stained with 0.2 ug Mouse IgM Isotype Control (MOPC-104E) (65243-1-Ig) and CoraLite®647-conjugated AffiniPure F(ab')2 Fragment Goat Anti-Mouse IgG (H+L) (SA00014-8) (red), or CoraLite®647-conjugated AffiniPure F(ab')2 Fragment Goat Anti-Mouse IgG (H+L) (SA00014-8) alone (black dashed), or unstained (blue). Cells were not fixed. Lymphocytes were gated.
FC experiment of human peripheral blood platelets using 65243-1-Ig
100ul human platelet-rich plasma (diluted 1:2 in PBS) was surface stained with 0.2 ug Mouse IgM Isotype Control (65243-1-Ig, Clone: MOPC-104E) (red) or Anti-Human CD36 (65248-1-Ig) (green) or unstained (blue) and CoraLite®647-conjugated F(ab')2 Fragment Goat Anti-Mouse IgG (H+L) (SA00014-8). Cells were not fixed.
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
IgM Isotype Control Monoclonal antibody
Catalog Number
65243-1-Ig
Citations
-
Dilutions
Applications
FC
Reactivity
n/a
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
