Cardinal Red™ Mouse IgG2b Isotype Control (MPC-11)

IgG2b Isotype Control Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG2b

Reactivity

Applications

FC

Conjugate

Cardinal Red™ Fluorescent Dye

CloneNo.

MPC-11

Cat no : CR-65128

Synonyms

gamma2b, IgG2b, Igh-3, IGHG2B

---

Validation Data Gallery

• 

  FC experiment of human PBMCs using CR-65128

  1X10^6 human PBMCs were surface stained with 0.2 ug Cardinal Red™ Anti-Mouse IgG2b Isotype Control (CR-65128, Clone:MPC-11) (red), 5 ul CR Anti-Human CD4 (green), or unstained (blue). Cells were not fixed. Lymphocytes were gated.

---

Tested Applications

Positive FC detected in

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CR-65128 targets IgG2b Isotype Control in FC applications and shows reactivity with samples.

• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: N/A
• Full Name: immunoglobulin heavy chain 3 (serum IgG2b)
• Gene Symbol: Igh-3
• Gene ID (NCBI): 16016
• Conjugate: Cardinal Red™ Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 592 nm / 611 nm
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

The MPC-11 immunoglobulin is useful as an isotype-matched control. The MPC-11 immunoglobulin has an unknown binding specificity and is used as an isotype control for mouse IgG2b antibodies. This antibody has been quality-tested for flow cytometry as negative control.