1X10^6 human PBMCs were surface stained with 0.2 ug Mouse IgG2b Isotype Control (65128-1-Ig, Clone:MPC-11) (blue), or Anti-Human CD4 (red) followed by CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 or unstained (black). Cells were not fixed. Lymphocytes were gated.
1X10^6 human PBMCs were surface stained with 0.2 ug Mouse IgG2b Isotype Control (65128-1-Ig, Clone:MPC-11) (blue), or Anti-Human CD4 (red) followed by CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 or unstained (black). Cells were not fixed. Lymphocytes were gated.
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
Product Information
65128-1-Ig targets IgG2b Isotype Control in FC applications and shows reactivity with n/a samples.
Store at 2-8°C. Stable for one year after shipment.
Background Information
The MPC-11 immunoglobulin is useful as an isotype-matched control. The MPC-11 immunoglobulin has an unknown binding specificity and is used as an isotype control for mouse IgG2b antibodies. This antibody has been quality-tested for flow cytometry as negative control.
Protocols
Product Specific Protocols
FC protocol for IgG2b Isotype Control antibody 65128-1-Ig
1X10^6 human PBMCs were surface stained with 0.2 ug Mouse IgG2b Isotype Control (65128-1-Ig, Clone:MPC-11) (blue), or Anti-Human CD4 (red) followed by CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 or unstained (black). Cells were not fixed. Lymphocytes were gated.
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
IgG2b Isotype Control Monoclonal antibody
Catalog Number
65128-1-Ig
Citations
-
Dilutions
Applications
FC
Reactivity
n/a
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
