APC Mouse IgG2b Isotype Control (MPC-11)

IgG2b Isotype Control Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG2b

Reactivity

and More (1)

Applications

FC

Conjugate

APC Fluorescent Dye

Publications

3

CloneNo.

MPC-11

Cat no : APC-65128

Synonyms

APC Mouse IgG2b Isotype Control, IgG2b, IgG2b Isotype Control

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Validation Data Gallery

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  FC experiment of human PBMCs using APC-65128

  1X10^6 human PBMCs were surface stained with 0.2 ug APC Anti-Mouse IgG2b Isotype Control (APC-65128, Clone:MPC-11) (red), APC Anti-Human CD20 (black), or unstained (blue). Cells were not fixed. Lymphocytes were gated.

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

• FC: See 3 publications below

Product Information

APC-65128 targets IgG2b Isotype Control in FC applications and shows reactivity with samples.

• Cited Reactivity: human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: N/A
• Full Name: immunoglobulin heavy chain 3 (serum IgG2b)
• Gene Symbol: IgG2b Isotype Control
• Gene ID (NCBI): 16016
• RRID: AB_2883011
• Conjugate: APC Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 650 nm / 660 nm
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

The MPC-11 immunoglobulin is useful as an isotype-matched control. The MPC-11 immunoglobulin has an unknown binding specificity and is used as an isotype control for mouse IgG2b antibodies. This antibody has been quality-tested for flow cytometry as negative control.

Publications

Species	Application	Title
human	FC	MedComm (2020); CircMALAT1 promotes cancer stem-like properties and chemoresistance via regulating Musashi-2/c-Myc axis in esophageal squamous cell carcinoma; Authors - Zitong Zhao
	FC	Eur J Pharmacol; PPARγ agonist pioglitazone enhances colorectal cancer immunotherapy by inducing PD-L1 autophagic degradation; Authors - Xiao Jia
human	FC	Auris Nasus Larynx; Simultaneous disturbance of NHE1 and LOXL2 decreases tumorigenicity of head and neck squamous cell carcinoma; Authors - Yuji Hayashi