CoraLite® Plus 647 Anti-Human IL-7Ra/CD127 Rabbit Recombinant Antibody

IL-7Ra/CD127 Uni-rAb^TM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

240749H2

Cat no : CL647-98074

Synonyms

CD127, 240749H2, IL 7RA, IL 7R subunit alpha, IL 7R alpha

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Validation Data Gallery

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  FC experiment of human PBMCs using CL647-98074

  1x10^6 human PBMCs were surface stained with PE Anti-Human CD3 and 5 ul CoraLite® Plus 647 Anti-Human IL-7Ra/CD127 Rabbit Recombinant Antibody (CL647-98074, Clone: 240749H2), or CoraLite® Plus 647 Rabbit IgG Isotype Control Recombinant Antibody (CL647-98136, Clone: 240953C9). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

• 

  FC experiment of human PBMCs using CL647-98074

  1x10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 647 Anti-Human IL-7Ra/CD127 Rabbit Recombinant Antibody (CL647-98074, Clone: 240749H2) (red), or CoraLite® Plus 647 Rabbit IgG Isotype Control Recombinant Antibody (CL647-98136, Clone: 240953C9) (blue). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

CL647-98074 targets IL-7Ra/CD127 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: interleukin 7 receptor
• Calculated Molecular Weight: 52 kDa
• GenBank Accession Number: AAC83204.1
• Gene Symbol: CD127
• Gene ID (NCBI): 3575
• Conjugate: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 654 nm / 674 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD127, also known as IL-7R subunit alpha (IL-7RA), is a type I membrane glycoprotein expressed on thymocytes, B cell precursors, most T cells, and some lymphoid and myeloid cells. IL-7R is a heterodimer composed of CD127 and the common-γ chain receptor (CD132). IL-7R plays critical roles in lymphocyte development and homeostasis. CD127 can also act as a receptor for thymic stromal lymphopoietin (TSLP).