Anti-Human IL-7Ra/CD127 Rabbit Recombinant Antibody

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  FC experiment of human PBMCs using 98074-1-RR

  1x10^6 human PBMCs were stained with CoraLite® Plus 647 Anti-Human CD3 (OKT3) Mouse IgG2a Recombinant Antibody (CL647-65569, Clone: OKT3) and 0.25 ug Anti-Human IL-7Ra/CD127 Rabbit Recombinant Antibody (98074-1-RR, Clone:240749H2), or Isotype Control. Cells were not fixed.

• 

  FC experiment of human PBMCs using 98074-1-RR

  1x10^6 human PBMCs were stained with 0.25 ug Anti-Human IL-7Ra/CD127 Rabbit Recombinant Antibody (98074-1-RR, Clone:240749H2) (red), or Isotype Control(blue). Cells were not fixed.

• 

  FC experiment of human PBMCs using 98074-1-RR

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human IL-7Ra/CD127 Rabbit Recombinant Antibody (98074-1-RR, Clone:240749H2) and PE-Conjugated Goat Anti-Rabbit IgG(H+L), and 0.25 ug CoraLite® Plus 647 Anti-Human CD3 (OKT3) Mouse IgG2a Recombinant Antibody (CL647-65569, Clone: OKT3), and 0.25 ug Isotype Control. Cells were not fixed.

• 

  Affinity and Kinetic Characterization of 98074-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98074-1-RR against Human IL-7Ra/CD127 were performed. The affinity constant is 4.57 nM.

IL-7Ra/CD127 Uni-rAb^TM Recombinant Antibody for FC

Cat No. 98074-1-RR

Clone No.240749H2

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

CD127, 240749H2, IL 7RA, IL 7R subunit alpha, IL 7R alpha

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

98074-1-RR targets IL-7Ra/CD127 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: interleukin 7 receptor
• Calculated Molecular Weight: 52 kDa
• GenBank Accession Number: AAC83204.1
• Gene Symbol: CD127
• Gene ID (NCBI): 3575
• ENSEMBL Gene ID: ENSG00000168685
• RRID: AB_3672221
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P16871-1(I66T, V138I)
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2 - 8°C. Stable for one year after shipment.

Background Information

CD127, also known as IL-7R subunit alpha (IL-7RA), is a type I membrane glycoprotein expressed on thymocytes, B cell precursors, most T cells, and some lymphoid and myeloid cells. IL-7R is a heterodimer composed of CD127 and the common-γ chain receptor (CD132). IL-7R plays critical roles in lymphocyte development and homeostasis. CD127 can also act as a receptor for thymic stromal lymphopoietin (TSLP).