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Species Reactivity
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Species Reactivity
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Conjugate
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CoraLite® Plus 647 Rabbit IgG Isotype Control Recombinant Antibody

IgG Isotype Control Uni-rAbTM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

n/a

Applications

FC

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

240953C9

Cat no : CL647-98136

Synonyms

Rabbit IgG Isotype Control, Rabbit IgG Isotype, Rabbit IgG, 240953C9

Validation Data Gallery

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  • Flow cytometry (FC) experiment of human peripheral blood leukocytes using CoraLite® Plus 647 Rabbit IgG Isotype Control Reco (CL647-98136)

    FC experiment of human peripheral blood leukocytes using CL647-98136

    1x10^6 human peripheral blood leukocytes were unstained(left, blue in histogram) or surface stained with 0.25 ug CoraLite® Plus 647 Rabbit IgG Isotype Control Recombinant Antibody (CL647-98136, Clone:240953C9)(mid, red in histogram), or 0.25 ug CoraLite® Plus 647 Anti-Human CD45 Rabbit Recombinant Antibody (CL647-98117, Clone: 241670E10) (right, green in histogram). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

Tested Applications

Positive FC detected in

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL647-98136 targets IgG Isotype Control in FC applications and shows reactivity with n/a samples.

Tested Reactivity n/a
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide
Full Name IgG Isotype Control
Gene Symbol
Gene ID (NCBI)
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission Maxima Wavelengths654 nm / 674 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

This product is an isotype control antibody, which is used to estimate the non-specific binding of target primary antibodies due to Fc receptor binding or other protein-protein interactions. An isotype control antibody should have the same immunoglobulin type and be used at the same concentration as the test antibody.

Protocols

Product Specific Protocols
FC protocol for CL Plus 647 IgG Isotype Control antibody CL647-98136Download protocol
Standard Protocols
Click here to view our Standard Protocols