CoraLite® Plus 405 Anti-Human IL-7 (1C11B11)

IL-7 Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

FC (Intra)

Conjugate

CoraLite® Plus 405 Fluorescent Dye

CloneNo.

1C11B11

Cat no : CL405-65281

Synonyms

IL7, IL-7, interleukin 7, Interleukin-7, 1C11B11, IL 7

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Validation Data Gallery

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  FC experiment of human PBMCs using CL405-65281

  1X10^6 LPS and Brefeldin A treated human PBMCs were intracellularly stained with 0.2 ug CoraLite® Plus 405 Anti-Human IL-7 (CL405-65281, Clone:1C11B11) or Mouse IgG1 Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). Monocytes were gated.

• 

  FC experiment of human PBMCs using CL405-65281

  1X10^6 LPS and Brefeldin A treated human PBMCs were intracellularly stained with 0.2 ug CoraLite® Plus 405 Anti-Human IL-7 (CL405-65281, Clone:1C11B11) (red) or Mouse IgG1 Isotype Control (blue), or unactivated and Brefeldin A treated human PBMCs were intracellularly stained with 0.2 ug CoraLite® Plus 405 Anti-Human IL-7 (CL405-65281, Clone:1C11B11) (black, dashed line). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). Monocytes were gated.

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Tested Applications

• Positive FC (Intra) detected in: LPS and Brefeldin A treated human PBMCs
• Positive FC detected in: LPS and Brefeldin A treated human PBMCs

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL405-65281 targets IL-7 in FC (Intra) applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: IL-7 fusion protein HZ-1281
• Full Name: interleukin 7
• Calculated Molecular Weight: 20 kDa
• GenBank Accession Number: BC047698
• Gene Symbol: IL-7
• Gene ID (NCBI): 3574
• Conjugate: CoraLite® Plus 405 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 399 nm / 422 nm
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

Interleukin-7 (IL-7) is a cytokine involved in B and T cell development. It plays an active role in the development, survival, maintaining and restoring homeostasis of mature T lymphocytes and is a key regulator of the commitment, survival, proliferation and maturation of B cells during development. Furthermore, IL-7 can improve the antiviral function and expansion of natural killer (NK) cells and regulate the development and differentiation of dendritic cells. IL-7 has also been reported as a regulator of the development of central nervous system and myogenesis and skeletal muscle cell migration. (PMID: 29663382; 29655570; 29449560)