PE Anti-Mouse IL-4 (11B11)

IL-4 Monoclonal Antibody for FC (Intra)

Host / Isotype

Rat / IgG1, kappa

Reactivity

Mouse and More (1)

Applications

FC (Intra)

Conjugate

PE Fluorescent Dye

CloneNo.

11B11

Cat no : PE-65074

Synonyms

IL-4, interleukin 4, 11B11, Il 4, Il4



Tested Applications

Positive FC detected inPMA and ionomycin stimulated Th2-polarized splenocytes

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

FCSee 1 publications below

Product Information

PE-65074 targets IL-4 in FC (Intra) applications and shows reactivity with Mouse samples.

Tested Reactivity Mouse
Cited Reactivitymouse
Host / Isotype Rat / IgG1, kappa
Class Monoclonal
Type Antibody
Immunogen Partially purified native mouse IL-4
Full Name interleukin 4
GenBank Accession NumberBC027514
Gene Symbol IL-4
Gene ID (NCBI) 16189
Conjugate PE Fluorescent Dye
Excitation/Emission Maxima Wavelengths496 nm, 565 nm / 578 nm
Form Liquid
Purification MethodAffinity purification
Storage Buffer PBS with 0.1% sodium azide and 0.5% BSA.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

Interleukin-4 (IL-4), a member of the α-helical cytokine family, is produced by activated CD4+ T cells, basophils, and mast cells. It promotes the proliferation and differentiation of antigen presenting cells. IL-4 also plays a pivotal role in antibody isotype switching and stimulates the production of IgE. This cytokine has been applied in the treatment of autoimmune disorder like multiple myeloma, cancer, psoriasis, and arthritis. IL-4 has also been extensively applied to inhibit detrimental effect of Th1. It may promote the growth of epithelial tumors by mediating increased proliferation and survival. (PMID: 24489573;3049907;21663408)

Protocols

Product Specific Protocols
FC protocol for PE IL-4 antibody PE-65074Download protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseFC

J Ethnopharmacol

Daqing formula ameliorated allergic asthma and airway dysbacteriosis in mice challenged with ovalbumin and ampicillin

Authors - Li-Ren Wu