PE Anti-Mouse IL-4 (11B11)

IL-4 Monoclonal Antibody for FC (Intra)

Host / Isotype

Rat / IgG1, kappa

Reactivity

Mouse and More (1)

Applications

FC (Intra)

Conjugate

PE Fluorescent Dye

Publications

1

CloneNo.

11B11

Cat no : PE-65074

Synonyms

IL-4, interleukin 4, 11B11, Il 4, Il4

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Validation Data Gallery

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  FC experiment of C57BL/6 Th2-polarized splenocytes using PE-65074

  1X10^6 unstimulated or PMA and ionomycin stimulated (in the presence of protein transport inhibitors) Th2-polarized splenocytes were surface stained 0.2 ug CoraLite® Plus 647 Anti-Mouse CD4 (GK1.5). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C), and then intracellularly stained with 0.125 ug PE Anti-Mouse IL-4 (PE-65074, Clone: 11B11).

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Tested Applications

• Positive FC detected in: PMA and ionomycin stimulated Th2-polarized splenocytes

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

• FC: See 1 publications below

Product Information

PE-65074 targets IL-4 in FC (Intra) applications and shows reactivity with Mouse samples.

• Tested Reactivity: Mouse
• Cited Reactivity: mouse
• Host / Isotype: Rat / IgG1, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: Partially purified native mouse IL-4
• Full Name: interleukin 4
• GenBank Accession Number: BC027514
• Gene Symbol: IL-4
• Gene ID (NCBI): 16189
• Conjugate: PE Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 496 nm, 565 nm / 578 nm
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: PBS with 0.1% sodium azide and 0.5% BSA.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

Interleukin-4 (IL-4), a member of the α-helical cytokine family, is produced by activated CD4+ T cells, basophils, and mast cells. It promotes the proliferation and differentiation of antigen presenting cells. IL-4 also plays a pivotal role in antibody isotype switching and stimulates the production of IgE. This cytokine has been applied in the treatment of autoimmune disorder like multiple myeloma, cancer, psoriasis, and arthritis. IL-4 has also been extensively applied to inhibit detrimental effect of Th1. It may promote the growth of epithelial tumors by mediating increased proliferation and survival. (PMID: 24489573;3049907;21663408)

Publications

Species	Application	Title
mouse	FC	J Ethnopharmacol; Daqing formula ameliorated allergic asthma and airway dysbacteriosis in mice challenged with ovalbumin and ampicillin; Authors - Li-Ren Wu