CoraLite® Plus 488 Anti-Mouse IL-4 (11B11)

IL-4 Monoclonal Antibody for FC (Intra)

Host / Isotype

Rat / IgG1, kappa

Reactivity

Mouse

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

11B11

Cat no : CL488-65074

Synonyms

Il 4, Il4, IL-4, interleukin 4

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Validation Data Gallery

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  FC experiment of Mouse Th2-polarized splenocytes using CL488-65074

  1X10^6 unstimulated or PMA and ionomycin stimulated (in the presence of protein transport inhibitors) mouse Th2-polarized splenocytes were surface stained with 0.25 ug CoraLite® Plus 647 Anti-Mouse CD4 (GK1.5) (CL647-65104, Clone: GK1.5). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C), and then intracellularly stained with 0.25 ug CoraLite® Plus 488 Anti-Mouse IL-4 (CL488-65074, Clone:11B11).

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Tested Applications

• Positive FC (Intra) detected in: PMA and ionomycin stimulated mouse Th2-polarized splenocytes
• Positive FC detected in: PMA and ionomycin stimulated mouse Th2-polarized splenocytes

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-65074 targets IL-4 in FC (Intra) applications and shows reactivity with Mouse samples.

• Tested Reactivity: Mouse
• Host / Isotype: Rat / IgG1, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: Partially purified native mouse IL-4
• Full Name: interleukin 4
• GenBank Accession Number: BC027514
• Gene Symbol: IL-4
• Gene ID (NCBI): 16189
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

Interleukin-4 (IL-4), a member of the α-helical cytokine family, is produced by activated CD4+ T cells, basophils, and mast cells. It promotes the proliferation and differentiation of antigen presenting cells. IL-4 also plays a pivotal role in antibody isotype switching and stimulates the production of IgE. This cytokine has been applied in the treatment of autoimmune disorder like multiple myeloma, cancer, psoriasis, and arthritis. IL-4 has also been extensively applied to inhibit detrimental effect of Th1. It may promote the growth of epithelial tumors by mediating increased proliferation and survival. (PMID: 24489573;3049907;21663408)

Related Products

Flow Cytometry Perm Buffer (10X)

PF00011-C