APC Anti-Mouse IL-4 (11B11)

• 

  FC experiment of C57BL/6 Th2-polarized splenocytes using APC-65074

  1X10^6 unstimulated or PMA and ionomycin stimulated (in the presence of protein transport inhibitors) mouse Th2-polarized splenocytes were surface stained with 0.2 ug PE Anti-Mouse CD4 (GK1.5) (PE-65104, Clone: GK1.5). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C), and then intracellularly stained with 0.5 ug APC Anti-Mouse IL-4 (APC-65074, Clone:11B11).

IL-4 Monoclonal Antibody for FC (Intra)

Cat No. APC-65074

Clone No.11B11

Host / Isotype

Rat / IgG1, kappa

Reactivity

mouse

Applications

FC (Intra)

Synonyms

interleukin 4, Il4, Il 4, 11B11

---

Tested Applications

• Positive FC (Intra) detected in: PMA and ionomycin stimulated mouse Th2-polarized splenocytes

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

APC-65074 targets IL-4 in FC (Intra) applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rat / IgG1, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: Partially purified native mouse IL-4
• Full Name: interleukin 4
• GenBank Accession Number: BC027514
• Gene Symbol: IL-4
• Gene ID (NCBI): 16189
• RRID: AB_2935216
• Conjugate: APC Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 650 nm / 660 nm
• Form: Liquid
• Purification Method: Affinity purification
• UNIPROT ID: P07750
• Storage Buffer: PBS with 0.1% sodium azide and 0.5% BSA.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

Interleukin-4 (IL-4), a member of the α-helical cytokine family, is produced by activated CD4+ T cells, basophils, and mast cells. It promotes the proliferation and differentiation of antigen presenting cells. IL-4 also plays a pivotal role in antibody isotype switching and stimulates the production of IgE. This cytokine has been applied in the treatment of autoimmune disorder like multiple myeloma, cancer, psoriasis, and arthritis. IL-4 has also been extensively applied to inhibit detrimental effect of Th1. It may promote the growth of epithelial tumors by mediating increased proliferation and survival. (PMID: 24489573;3049907;21663408)