Anti-Human IL-12 (1E12H6)

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  FC experiment of human PBMCs using 65277-1-Ig

  1X10^6 LPS and Brefeldin A treated human PBMCs were surface stained with CoraLite ® Plus 750 Anti-Human HLA-DR then intracellularly stained with 0.2 ug CoraLite ® Plus 488 Anti-Human IL-12 (CL488-65277, Clone:1E12H6) or Mouse IgG1 Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). Monocytes were gated. This data was developed using the CoraLite ® Plus 488 conjugated antibody of 65277-1-Ig.

• 

  FC experiment of human PBMCs using 65277-1-Ig

  1X10^6 LPS and Brefeldin A treated human PBMCs were intracellularly stained with 0.2 ug CoraLite® Plus 488 Anti-Human IL-12 (CL488-65277, Clone:1E12H6) (red) or Mouse IgG1 Isotype Control (blue), or unstimulated and Brefeldin A treated human PBMCs were intracellularly stained with 0.2 ug CoraLite® Plus 488 Anti-Human IL-12 (CL488-65277, Clone:1E12H6) (black, dashed line). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). Monocytes were gated. This data was developed using the CoraLite ® Plus 488 conjugated antibody of 65277-1-Ig.

IL-12 Monoclonal Antibody for FC (Intra)

Cat No. 65277-1-Ig

Clone No.1E12H6

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

FC (Intra)

Synonyms

1E12H6, CLMF, CLMF p35, IL 12 subunit p35, IL 12A

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Tested Applications

• Positive FC (Intra) detected in: LPS and Brefeldin A treated human PBMCs

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

65277-1-Ig targets IL-12 in FC (Intra) applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: IL-12 fusion protein HZ-1256
• Full Name: interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35)
• Calculated Molecular Weight: 25 kDa
• GenBank Accession Number: BC104982
• Gene Symbol: IL-12A
• Gene ID (NCBI): 3592
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: PBS with 0.09% sodium azide , pH 7.3
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

IL-12 is a heterodimeric cytokine composed of two glycosylated and disulfide-linked subunits (p40 cysteine-linked to p35). IL-12 is a potent regulator of cell mediated immune responses and it induces IFN-gamma production by NK and T cells. It is produced by activated monocytes/macrophage cells, B lymphocytes, and connective tissue type mast cells (PMID: 24821971; 7612223).