• HEK293 expressed
  • Endotoxin-free
  • Animal-component free

HumanKine® recombinant human IL-12 protein


0.8-4 ng/mL EC50

Specific Activity

>3.00 x 106 IU/mg

Species Reactivity




Cat no : HZ-1256


CLMF, CLMF p35, IL 12 subunit p35, IL 12A, IL12, IL-12, IL12A, Interleukin 12 subunit alpha, NFSK, NKSF1, P35

Technical Specifications

GeneID 3592
Species Human
Expression HEK293
Activity(Primary) 0.8-4 ng/mL EC50
Specific Activity>3.00 x 106 IU/mg
Purity >95%
Endotoxin<1 EU/μg
Accession NumberP29459 (p35 subunit) P29460 (p40 subunit)
Molecular Mass 57 kDa, heterodimer, glycosylated
Formulation 1x PBS, See Certificate of Analysis for details
Species Reactivity human,mouse

Stability and Reconstitution

Stability and StorageProduct FormTemperature ConditionsStorage Time
(From Date of Receipt)
Lyophilized-20°C to -80°CUntil Expiry Date
LyophilizedRoom Temperature2 weeks
Reconstituted as per CofA-20°C to -80°C6 months
Reconstituted as per CofA4°C1 week
Avoid repeated freeze-thaw cycles.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 1xPBS pH 7.4 containing 0.1% endotoxin-free recombinant human serum albumin (HSA).


IL-12 is a heterodimeric cytokine composed of two glycosylated and disulfide-linked subunits (p40 cysteine-linked to p35). IL-12 is a potent regulator of cell mediated immune responses and it induces IFN-gamma production by NK and T cells. It is produced by activated monocytes/macrophage cells, B lymphocytes, and connective tissue type mast cells (PMID: 24821971; 7612223).


CLMF, CLMF p35, IL 12 subunit p35, IL 12A, IL12, IL-12, IL12A, Interleukin 12 subunit alpha, NFSK, NKSF1, P35



Nat Commun

Extracellular mRNA transported to the nucleus exerts translation-independent function.

Authors - Takeshi Tomita

J Mol Cell Cardiol

Systemic transplantation of allogenic fetal membrane-derived mesenchymal stem cells suppresses Th1 and Th17 T cell responses in experimental autoimmune myocarditis.

Authors - MakikoOhshima

Front Immunol

Tim-3 Expression Causes NK Cell Dysfunction in Type 2 Diabetes Patients.

Authors - Hui Wang

Immunol Cell Biol

Regulation of human Th9 differentiation by type I interferons and IL-21.

Authors - Michael TWong

J Immunol Methods

In vitro Th1 and Th2 cell polarization is severely influenced by the initial ratio of naïve and memory CD4+ T cells.

Authors - LarsBlom

Cell Rep

In vivo G-CSF treatment activates the GR-SOCS1 axis to suppress IFN-γ secretion by natural killer cells

Authors - Xiangyu Zhao


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


CHELI (Verified Customer) (01-10-2022)

Performed immune cells stimulation with this recombinant IL-12 and the activation was robust. it worked very well!

  • Applications: Cell culture