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  • HEK293 expressed
  • Endotoxin-free
  • Animal-component free

HumanKine® recombinant human IL-12 protein

Activity(Primary)

0.8-4 ng/mL

Specific Activity

minimally 3.00 x 106 IU/mg

Species Reactivity

human,mouse

Purity

>95%

Cat no : HZ-1256

Synonyms

CLMF, CLMF p35, IL 12 subunit p35, IL 12A, IL12, IL-12, IL12A, Interleukin 12 subunit alpha, NFSK, NKSF1, P35

Validation Data Gallery

  • Purity of recombinant human IL-12 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

    Purity of recombinant human IL-12 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

  • Recombinant human IL-12 (HZ-1256) stimulates dose-dependent induction of alkaline phosphatase production in a HEK293 reporter cell line. Alkaline phosphatase production was assessed using pNPP as a chromogenic substrate. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 values range from 1-5 ng/mL.

    Recombinant human IL-12 (HZ-1256) stimulates dose-dependent induction of alkaline phosphatase production in a HEK293 reporter cell line. Alkaline phosphatase production was assessed using pNPP as a chromogenic substrate. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 values range from 1-5 ng/mL.

  • Proteintech IL-12 (HZ-1256) demonstrates equivalent EC50 and alkaline phosphatase induction to leading competitors. Recombinant human IL-12 stimulates dose-dependent induction of alkaline phosphatase production in a HEK293 reporter cell line. Alkaline phosphatase production was assessed using pNPP as a chromogenic substrate. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 values range from 1-5 ng/mL.

    Proteintech IL-12 (HZ-1256) demonstrates equivalent EC50 and alkaline phosphatase induction to leading competitors. Recombinant human IL-12 stimulates dose-dependent induction of alkaline phosphatase production in a HEK293 reporter cell line. Alkaline phosphatase production was assessed using pNPP as a chromogenic substrate. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 values range from 1-5 ng/mL.

Technical Specifications

GeneID 3592 and 3593
Species Human
Expression HEK293
Activity(Primary) 0.8-4 ng/mL
Specific Activityminimally 3.00 x 106 IU/mg
Purity >95%
Endotoxin<1 EU/μg
Accession NumberP29459 (p35 subunit) P29460 (p40 subunit)
Molecular Mass 34 and 42 kDa reduced, 57 kDa non-reduced, heterodimer, glycosylated
Formulation 1x PBS, See Certificate of Analysis for details
Species Reactivity human,mouse

Stability and Reconstitution

Stability and StorageProduct FormTemperature ConditionsStorage Time
(From Date of Receipt)
Lyophilized-20°C to -80°CUntil Expiry Date
LyophilizedRoom Temperature2 weeks
Reconstituted as per CofA-20°C to -80°C6 months
Reconstituted as per CofA4°C1 week
Avoid repeated freeze-thaw cycles.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein to 0.2 mg/mL in sterile 1x PBS pH 7.4 containing 0.1% endotoxin-free recombinant human serum albumin (HSA). Gently swirl or tap vial to mix.

Background

IL-12 is a heterodimeric cytokine composed of two glycosylated and disulfide-linked subunits (p40 cysteine-linked to p35). IL-12 is a potent regulator of cell mediated immune responses and it induces IFN-gamma production by NK and T cells. It is produced by activated monocytes/macrophage cells, B lymphocytes, and connective tissue type mast cells (PMID: 24821971; 7612223).

Synonyms

CLMF, CLMF p35, IL 12 subunit p35, IL 12A, IL12, IL-12, IL12A, Interleukin 12 subunit alpha, NFSK, NKSF1, P35

Publications

SpeciesTitle

Nat Commun

Extracellular mRNA transported to the nucleus exerts translation-independent function.

Authors - Takeshi Tomita
View Article
human

J Mol Cell Cardiol

Systemic transplantation of allogenic fetal membrane-derived mesenchymal stem cells suppresses Th1 and Th17 T cell responses in experimental autoimmune myocarditis.

Authors - MakikoOhshima
View Article
human

Front Immunol

Tim-3 Expression Causes NK Cell Dysfunction in Type 2 Diabetes Patients.

Authors - Hui Wang
View Article
human

Immunol Cell Biol

Regulation of human Th9 differentiation by type I interferons and IL-21.

Authors - Michael TWong
View Article
human,mouse

J Immunol Methods

In vitro Th1 and Th2 cell polarization is severely influenced by the initial ratio of naïve and memory CD4+ T cells.

Authors - LarsBlom
View Article
mouse

Cell Rep

In vivo G-CSF treatment activates the GR-SOCS1 axis to suppress IFN-γ secretion by natural killer cells

Authors - Xiangyu Zhao
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH

CHELI (Verified Customer) (01-10-2022)

Performed immune cells stimulation with this recombinant IL-12 and the activation was robust. it worked very well!

  • Applications: Cell culture