CoraLite® Plus 488-conjugated IKZF4 Recombinant antibody

• 

  IF Staining of U2OS using CL488-83471-5

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using CoraLite® Plus 488 IKZF4 antibody (CL488-83471-5, Clone: 240357D5 ) at dilution of 1:200.

• 

  FC experiment of HeLa using CL488-83471-5

  1x10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488-conjugated IKZF4 Recombinant antibody (CL488-83471-5, Clone:240357D5)(red), or 0.4 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

IKZF4 Uni-rAb^TM Recombinant Antibody for IF/ICC, FC (Intra)

Cat No. CL488-83471-5

Clone No.240357D5

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra)

Synonyms

ZNFN1A4, Zinc finger protein Eos, KIAA1782, IKZF 4, EOS

---

Tested Applications

• Positive IF/ICC detected in: U2OS cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-83471-5 targets IKZF4 in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: IKZF4 fusion protein Ag34767
• Full Name: IKAROS family zinc finger 4 (Eos)
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: BC160169
• Gene Symbol: IKZF4
• Gene ID (NCBI): 64375
• RRID: AB_3673255
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9H2S9
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

IKZF4 is a member of the Ikaros family transcription factors. The Ikaros family was originally designated to function in hematopoiesis, but it is only recently that studies have begun to show the relevance of this family in T helper cell differentiation. IKZF3 was preferentially expressed in TH17 cells and governs TH17 differentiation by silencing IL-2 expression. By investigating the dynamics of the TH17 regulatory network, IKZF4 was proposed by Yosef et al. as a composite in the negative regulatory module for TH17. (PMID: 24644282)