IKZF4 Recombinant antibody

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  WB analysis of K-562 using 83471-5-RR

  K-562 cells were subjected to SDS PAGE followed by western blot with 83471-5-RR (IKZF4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis using 83471-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83471-5-RR (IKZF4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IF Staining of U2OS using 83471-5-RR

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using IKZF4 antibody (83471-5-RR, Clone: 240357D5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red).

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  FC experiment of U2OS using 83471-5-RR

  1x10^6 U2OS cells were intracellularly stained with 0.25 ug IKZF4 Recombinant antibody (83471-5-RR, Clone:240357D5) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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  Affinity and Kinetic Characterization of 83471-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83471-5-RR against Human IKZF4 were performed. The affinity constant is 21.3 nM.

IKZF4 Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Cat No. 83471-5-RR

Clone No.240357D5

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), ELISA

Synonyms

ZNFN1A4, Zinc finger protein Eos, KIAA1782, EOS, 240357D5

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Tested Applications

• Positive WB detected in: Raji cells, K-562 cells, HepG2 cells
• Positive IF/ICC detected in: U2OS cells
• Positive FC (Intra) detected in: U2OS cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

83471-5-RR targets IKZF4 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: IKZF4 fusion protein Ag34767
• Full Name: IKAROS family zinc finger 4 (Eos)
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: BC160169
• Gene Symbol: IKZF4
• Gene ID (NCBI): 64375
• RRID: AB_3671101
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q9H2S9
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IKZF4 is a member of the Ikaros family transcription factors. The Ikaros family was originally designated to function in hematopoiesis, but it is only recently that studies have begun to show the relevance of this family in T helper cell differentiation. IKZF3 was preferentially expressed in TH17 cells and governs TH17 differentiation by silencing IL-2 expression. By investigating the dynamics of the TH17 regulatory network, IKZF4 was proposed by Yosef et al. as a composite in the negative regulatory module for TH17. (PMID: 24644282)