IGFBP2 Recombinant antibody, PBS Only (Capture)

IGFBP2 Recombinant Antibody for WB, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

240227D4

Cat no : 83283-3-PBS

Synonyms

IBP-2, IBP2, IBP 2, BP2, BP 2

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Validation Data Gallery

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  WB analysis using 83283-3-RR (same clone as 83283-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83283-3-RR (IGFBP2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83283-3-PBS in a different storage buffer formulation.

• 

  Sandwich ELISA standard curve of MP00322-2

  Sandwich ELISA standard curve of MP00322-2, human IGFBP2 Recombinant Matched Antibody Pair - PBS only. 83283-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0823. 83283-2-PBS was HRP conjugated as the detection antibody. Range: 78.1-5000 pg/mL.

• 

  Sample test of MP00322-2

  Urine of eight individual healthy human donors was measured. The IGFBP2 concentration of detected samples was determined to be 6251.0 pg/mL with a range of 306.2 - 19423.8 pg/mL.

• 

  Sample test of MP00322-2

  MCF-7 human breast cancer cells (5 x 10^6 cells/mL) were cultured in DMEM and 10% fetal bovine serum, 4 mM L-glutamine, 4500 mg/L glucose, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. An aliquot of the cell culture supernatants was removed, assayed for human IGFBP2, and measured 3750.9 pg/mL. MDA-MB-453 human breast carcinoma cells (5 x 10^6 cells/mL) were cultured in DMEM supplemented with 10% fetal bovine serum, 2.5 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. An aliquot of the cell culture supernatants was removed, assayed for human IGFBP2, and measured 9611.6 pg/mL.

• 

  Sample test of MP00322-2

  Serum of eight individual healthy human donors was measured. The IGFBP2 concentration of detected samples was determined to be 109.5 ng/mL with a range of 47.4 - 199.1 ng/mL.

• 

  Affinity and Kinetic Characterization of 83283-3-RR

  Biolayer interferometry (BLl) kinetic assays of 83283-3-RR against Human IGFBP2 were performed. The affinity constant is 1.85 nM.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83283-3-PBS targets IGFBP2 as part of a matched antibody pair:

MP00322-2: 83283-3-PBS capture and 83283-2-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: IGF binding protein 2
• Calculated Molecular Weight: 35 kDa
• GenBank Accession Number: NM_000597.3
• Gene Symbol: IGFBP2
• Gene ID (NCBI): 3485
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.