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IGFBP2 Recombinant antibody

IGFBP2 Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240227D4

Cat no : 83283-3-RR

Synonyms

BP2, IBP 2, IBP2, IGF binding protein 2, IGF BP53, IGFBP 2, IGFBP2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using IGFBP2 Recombinant antibody (83283-3-RR)

    WB analysis using 83283-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83283-3-RR (IGFBP2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity experiments of 83283-3-RR

    Affinity experiments of 83283-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83283-3-RR against Human IGFBP2 were performed. The affinity constant is 1.85 nM.

Tested Applications

Positive WB detected inSH-SY5Y cells, T-47D cells, human plasma sample

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83283-3-RR is IGFBP2 Fusion Protein expressed in E. coli.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein
Full Name IGF binding protein 2
Calculated Molecular Weight35 kDa
Observed Molecular Weight 33-36 kDa
GenBank Accession NumberNM_000597.3
Gene Symbol IGFBP2
Gene ID (NCBI) 3485
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Insulin-like growth factors (IGFs) and their binding proteins (IGFBPs) have important metabolic roles in the human body. IGFBP-2 is a 34 kDa IGFBP protein with degraded fragments of 24 kDa, 19 kDa, and 15 kDa. The levels of IGFBP2 are elevated during the progression of many human cancers. IGFBP2 is expressed in invasive PAc, whereas its expression in HG-PIN is low. It can be as an Immunohistochemical Marker for Prostatic Adenocarcinoma.

Protocols

Product Specific Protocols
WB protocol for IGFBP2 antibody 83283-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols