IGFBP2 Recombinant antibody

IGFBP2 Uni-rAb^TM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240227D4

Cat no : 83283-3-RR

Synonyms

BP2, IBP 2, IBP2, IGF binding protein 2, IGF BP53, IGFBP 2, IGFBP2

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Validation Data Gallery

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  WB analysis using 83283-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83283-3-RR (IGFBP2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 83283-3-RR

  Biolayer interferometry (BLl) kinetic assays of 83283-3-RR against Human IGFBP2 were performed. The affinity constant is 1.85 nM.

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Tested Applications

• Positive WB detected in: SH-SY5Y cells, T-47D cells, human plasma sample

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83283-3-RR is IGFBP2 Fusion Protein expressed in E. coli.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: IGF binding protein 2
• Calculated Molecular Weight: 35 kDa
• Observed Molecular Weight: 33-36 kDa
• GenBank Accession Number: NM_000597.3
• Gene Symbol: IGFBP2
• Gene ID (NCBI): 3485
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Insulin-like growth factors (IGFs) and their binding proteins (IGFBPs) have important metabolic roles in the human body. IGFBP-2 is a 34 kDa IGFBP protein with degraded fragments of 24 kDa, 19 kDa, and 15 kDa. The levels of IGFBP2 are elevated during the progression of many human cancers. IGFBP2 is expressed in invasive PAc, whereas its expression in HG-PIN is low. It can be as an Immunohistochemical Marker for Prostatic Adenocarcinoma.