IGF2 Recombinant antibody

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  WB analysis of HuH-7 using 84643-1-RR

  HuH-7 cells were subjected to SDS PAGE followed by western blot with 84643-1-RR (IGF2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 84643-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84643-1-RR against Human IGF2 were performed. The affinity constant is 0.159 nM.

IGF2 Recombinant Antibody for WB, ELISA

Cat No. 84643-1-RR

Clone No.241746D3

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

proIGF-II, PP1446, Insulin-like growth factor II Ala-25 Del, IGF-II, IGF II

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Tested Applications

• Positive WB detected in: HuH-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84643-1-RR targets IGF2 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: IGF2 fusion protein HZ-1161
• Full Name: insulin-like growth factor 2 (somatomedin A)
• Observed Molecular Weight: 20 kDa
• Gene Symbol: IGF2
• Gene ID (NCBI): 3481
• RRID: AB_3672102
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P01344
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Insulin-like growth factor 2 (IGF2) is a crucial protein that plays a significant role in regulating growth, particularly during normal fetal development, and is also often dysregulated during tumorigenesis. IGF2 is one of the main ligands of the type 1 IGF receptor (IGF-1R), a tyrosine kinase receptor that transmits signals upon binding with IGF-II. IGF2 is predominantly secreted via the placenta during pregnancy and, after birth, primarily by hepatocytes, with its secretion being independent of growth hormone (GH) secretion, unlike IGF-I.