HumanKine® recombinant human pro IGF-II protein

• HEK293 expressed
• Endotoxin-free
• Animal-component free

Activity(Primary)

12-80 ng/mL

Species Reactivity

human,mouse

Purity

>95%

Publications

2

Cat no : HZ-1161

Synonyms

C11orf43, FLJ22066, FLJ44734, IGF II, IGF2, INSIGF, Insulin like growth factor II, pp9974, Somatomedin A

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Validation Data Gallery

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  Purity of recombinant human Pro-IGF-II was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

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  The activity was determined by the dose-dependent stimulation of the proliferation of MCF-7 cells (human breast cancer cell line) using the Promega CellTiter96® Aqueous Non-Radioactive Cell Proliferation Assay.

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Technical Specifications

• GeneID: 3481
• Species: Human
• Expression: HEK293
• Activity(Primary): 12-80 ng/mL
• Purity: >95%
• Endotoxin: <1 EU/μg
• Accession Number: P01344
• Molecular Mass: 25 kDa reduced and non-reduced, monomer, glycosylated
• Formulation: 10 mM Tris-HCl pH 7.4 + 350 mM NaCl, See Certificate of Analysis for details
• Species Reactivity: human,mouse

Stability and Reconstitution

Stability and Storage	Product Form	Temperature Conditions	Storage Time (From Date of Receipt)
	Lyophilized	-20°C to -80°C	Until Expiry Date
	Lyophilized	Room Temperature	2 weeks
	Reconstituted as per CofA	-20°C to -80°C	6 months
	Reconstituted as per CofA	4°C	1 week
	Avoid repeated freeze-thaw cycles.

Reconstitution

Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein to 0.2 mg/mL in sterile 1x PBS pH 7.4 containing 0.1% endotoxin-free recombinant human serum albumin (HSA). Gently swirl or tap vial to mix.

Background

IGF2 is a hormone involved in the regulation of cell proliferation, growth, migration, differentiation, and survival. IGF2 is mostly expressed in early embryonic and fetal development. Adult IGF2 expression occurs in the liver and epithelial cells lining the surface of the brain. IGF2 can also be detected in plasma (PMID: 24593700; 23257688)

Synonyms

C11orf43, FLJ22066, FLJ44734, IGF II, IGF2, INSIGF, Insulin like growth factor II, pp9974, Somatomedin A

Publications

Species	Title
mouse	Stem Cells; Pro-Insulin-Like Growth Factor-II Ameliorates Age-Related Inefficient Regenerative Response by Orchestrating Self-Reinforcement Mechanism of Muscle Regeneration.; Authors - Madoka Ikemoto-Uezumi
human	J Clin Endocrinol Metab; Paracrine Pathways in Uterine Leiomyoma Stem Cells Involve Insulinlike Growth Factor 2 and Insulin Receptor A.; Authors - Molly B Moravek