CoraLite®594-conjugated IFT88 Polyclonal antibody

• Featured Product
• KD/KO Validated

IFT88 Polyclonal Antibody for IF

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, Canine

Applications

IF

Conjugate

CoraLite®594 Fluorescent Dye

Cat no : CL594-13967

Synonyms

D13S1056E, DAF19, hTg737, IFT88, RP11 172H24.2, TG737, TPR repeat protein 10, TTC10

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Validation Data Gallery

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  IF Staining of MDCK using CL594-13967

  Immunofluorescent analysis of (4% PFA) fixed MDCK cells using CoraLite®594 IFT88 antibody (CL594-13967) at dilution of 1:200.

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  IF Staining of hTERT-RPE1 using CL594-13967

  Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using CoraLite®594 IFT88 antibody (CL594-13967) at dilution of 1:200, CoraLite® Plus 488 acetylated Tubulin(Lys40) antibody (CL488-66200, Clone: 7E5H8, green).

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Tested Applications

• Positive IF detected in: MDCK cells, hTERT-RPE1 cells

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-13967 targets IFT88 in IF applications and shows reactivity with human, mouse, rat, Canine samples.

• Tested Reactivity: human, mouse, rat, Canine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: IFT88 fusion protein Ag4980
• Full Name: intraflagellar transport 88 homolog (Chlamydomonas)
• Calculated Molecular Weight: 94 kDa
• Observed Molecular Weight: 88-95 kDa
• GenBank Accession Number: BC030776
• Gene Symbol: IFT88
• Gene ID (NCBI): 8100
• RRID: AB_2919801
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Intraflagellar transport (IFT), mediated by molecular motors and IFT particles, is an important transport process that occurs in the cilium and has been shown to be essential for the assembly and maintenance of cilia and flagella in many organisms. IFT88 (intraflagellar transport protein 88; also known as TG737 or TTC10) is a component of IFT particles and required for cilium biogenesis. Defects in IFT88/Tg737 lead to polycystic kidney disease (11062270). IFT88 localizes to spindle poles during mitosis and is required for spindle orientation in mitosis (21441926). This antibody was raised against the C-terminal region of human IFT88 and can detect the endogenous level of IFT88.