CoraLite®594-conjugated IFT140 Polyclonal antibody

• Featured Product
• KD/KO Validated

IFT140 Polyclonal Antibody for IF/ICC, IF-P

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, IF-P

Conjugate

CoraLite®594 Fluorescent Dye

Cat no : CL594-17460

Synonyms

WDTC2, KIAA0590, Intraflagellar transport protein 140 homolog

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Validation Data Gallery

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  IF Staining of mouse testis using CL594-17460

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using CoraLite®594 IFT140 antibody (CL594-17460) at dilution of 1:200.

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  IF Staining of mouse testis using CL594-17460

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using CoraLite®594 IFT140 antibody (CL594-17460) at dilution of 1:200.

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  IF Staining of hTERT-RPE1 using CL594-17460

  Immunofluorescent analysis of (-20°C Methanol) fixed hTERT-RPE1 cells using CoraLite®594 IFT140 antibody (CL594-17460) at dilution of 1:200, CoraLite® Plus 488 ARL13B antibody (CL488-17711, green).

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Tested Applications

• Positive IF-P detected in: mouse testis tissue
• Positive IF/ICC detected in: hTERT-RPE1 cells

Recommended dilution

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-17460 targets IFT140 in IF/ICC, IF-P applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: IFT140 fusion protein Ag11119
• Full Name: intraflagellar transport 140 homolog (Chlamydomonas)
• Calculated Molecular Weight: 1462 aa, 165 kDa
• GenBank Accession Number: BC035577
• Gene Symbol: IFT140
• Gene ID (NCBI): 9742
• RRID: AB_2919840
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

IFT140 is a subunit of intraflagellar transport complex A (IFT-A) which is involved in retrograde ciliary transport. RT-PCR analysis showed it is highly expressed in kidney, moderately in ovary, testis, prostate, and lung. IFT140 is localised to the base and tip of primary cilium. IFT140 has a pivotal role in development and function of ciliated cells, and mutations of IFT140 cause skeletal, renal, and retinal ciliopathies. It had been detected as a single band around 140-165 kDa in different reports. (PMID: 20368623, 22282595)