IFT140 Polyclonal antibody

• Featured Product
• KD/KO Validated

IFT140 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Publications

67

Cat no : 17460-1-AP

Synonyms

c305C8.4, c380F5.1, gs114, IFT140, KIAA0590, WDTC2

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Validation Data Gallery

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  WB analysis using 17460-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17460-1-AP (IFT140 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 17460-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 17460-1-AP (IFT140 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 17460-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 17460-1-AP (IFT140 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of rat testis using 17460-1-AP

  rat testis tissue were subjected to SDS PAGE followed by western blot with 17460-1-AP (IFT140 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of rat testis using 17460-1-AP

  IP Result of anti-IFT140 (IP:17460-1-AP, 4ug; Detection:17460-1-AP 1:300) with rat testis tissue lysate 4400ug.

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  IHC staining of mouse testis using 17460-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 17460-1-AP (IFT140 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human skeletal muscle using 17460-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 17460-1-AP (IFT140 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human skeletal muscle using 17460-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 17460-1-AP (IFT140 antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of C2C12 using 17460-1-AP

  Immunofluorescent analysis of (4% PFA) fixed C2C12 cells using IFT140 antibody (17460-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), acetylated Tubulin(Lys40) antibody (66200-1-Ig, Clone: 7E5H8, red).

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  IF Staining of mouse testis using 17460-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using IFT140 antibody (17460-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). DAPI (blue).

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  IF Staining of hTERT-RPE1 using 17460-1-AP

  Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using IFT140 antibody (17460-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), acetylated Tubulin(Lys40) antibody (66200-1-Ig, Clone: 7E5H8, red).

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Tested Applications

• Positive WB detected in: HeLa cells, rat testis tissue, HepG2 cells, mouse testis tissue
• Positive IP detected in: rat testis tissue
• Positive IHC detected in: mouse testis tissue, human skeletal muscle tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: C2C12 cells, hTERT-RPE1 cells, mouse testis tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:400-1:1600

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 3 publications below
• WB: See 20 publications below
• IHC: See 4 publications below
• IF: See 53 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Product Information

17460-1-AP targets IFT140 in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, zebrafish, canine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: IFT140 fusion protein Ag11119
• Full Name: intraflagellar transport 140 homolog (Chlamydomonas)
• Calculated molecular weight: 1462 aa, 165 kDa
• Observed molecular weight: 140 kDa
• GenBank accession number: BC035577
• Gene symbol: IFT140
• Gene ID (NCBI): 9742
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IFT140 is a subunit of intraflagellar transport complex A (IFT-A) which is involved in retrograde ciliary transport. RT-PCR analysis showed it is highly expressed in kidney, moderately in ovary, testis, prostate, and lung. IFT140 is localised to the base and tip of primary cilium. IFT140 has a pivotal role in development and function of ciliated cells, and mutations of IFT140 cause skeletal, renal, and retinal ciliopathies. It had been detected as a single band around 140-165 kDa in different reports. (PMID: 20368623, 22282595)

Publications

Species	Application	Title
human	IF	Nat Genet; Mutations in PLK4, encoding a master regulator of centriole biogenesis, cause microcephaly, growth failure and retinopathy.; Authors - Carol-Anne Martin
mouse	WB,IF	Cell; Dynamic Remodeling of Membrane Composition Drives Cell Cycle through Primary Cilia Excision.; Authors - Siew Cheng Phua
human	IF	Sci Adv; Ciliopathy protein HYLS1 coordinates the biogenesis and signaling of primary cilia by activating the ciliary lipid kinase PIPKIγ.; Authors - Chuan Chen
human	IF	Nat Commun; A distal centriolar protein network controls organelle maturation and asymmetry.; Authors - Lei Wang
mouse	WB	Nat Commun; Kinesin-2 and IFT-A act as a complex promoting nuclear localization of β-catenin during Wnt signalling.; Authors - Linh T Vuong
human	WB	Nat Commun; IFT proteins spatially control the geometry of cleavage furrow ingression and lumen positioning.; Authors - Nicolas Taulet