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IFT140 Polyclonal antibody
IFT140 Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (2)
Applications
WB, IP, IHC, IF, CoIP, ELISA
Conjugate
Unconjugated
Cat no : 17460-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HeLa cells, rat testis tissue, HepG2 cells, mouse testis tissue |
Positive IP detected in | rat testis tissue |
Positive IHC detected in | mouse testis tissue, human skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | C2C12 cells, hTERT-RPE1 cells, mouse testis tissue |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:6000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF) | IF : 1:400-1:1600 |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 3 publications below |
WB | See 20 publications below |
IHC | See 4 publications below |
IF | See 53 publications below |
IP | See 1 publications below |
CoIP | See 1 publications below |
Product Information
17460-1-AP targets IFT140 in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, zebrafish, canine |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | IFT140 fusion protein Ag11119 |
Full Name | intraflagellar transport 140 homolog (Chlamydomonas) |
Calculated molecular weight | 1462 aa, 165 kDa |
Observed molecular weight | 140 kDa |
GenBank accession number | BC035577 |
Gene symbol | IFT140 |
Gene ID (NCBI) | 9742 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
IFT140 is a subunit of intraflagellar transport complex A (IFT-A) which is involved in retrograde ciliary transport. RT-PCR analysis showed it is highly expressed in kidney, moderately in ovary, testis, prostate, and lung. IFT140 is localised to the base and tip of primary cilium. IFT140 has a pivotal role in development and function of ciliated cells, and mutations of IFT140 cause skeletal, renal, and retinal ciliopathies. It had been detected as a single band around 140-165 kDa in different reports. (PMID: 20368623, 22282595)
Protocols
Product Specific Protocols | |
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WB protocol for IFT140 antibody 17460-1-AP | Download protocol |
IHC protocol for IFT140 antibody 17460-1-AP | Download protocol |
IF protocol for IFT140 antibody 17460-1-AP | Download protocol |
IP protocol for IFT140 antibody 17460-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Nat Genet Mutations in PLK4, encoding a master regulator of centriole biogenesis, cause microcephaly, growth failure and retinopathy. | ||
Cell Dynamic Remodeling of Membrane Composition Drives Cell Cycle through Primary Cilia Excision. | ||
Sci Adv Ciliopathy protein HYLS1 coordinates the biogenesis and signaling of primary cilia by activating the ciliary lipid kinase PIPKIγ. | ||
Nat Commun A distal centriolar protein network controls organelle maturation and asymmetry. | ||
Nat Commun Kinesin-2 and IFT-A act as a complex promoting nuclear localization of β-catenin during Wnt signalling. | ||
Nat Commun IFT proteins spatially control the geometry of cleavage furrow ingression and lumen positioning. |