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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated ARL13B Polyclonal antibody

ARL13B Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, Canine

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-17711

Synonyms

ARL13B, ARL2 like protein 1, ARL2L1, ARL2-like protein 1, ADP-ribosylation factor-like protein 13B, ADP-ribosylation factor-like protein 2-like 1

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of hTERT-RPE1 cells using CoraLite® Plus 488-conjugated ARL13B Polyclonal an (CL488-17711)

    IF Staining of hTERT-RPE1 using CL488-17711

    Immunofluorescent analysis of (-20°C Methanol) fixed hTERT-RPE1 cells using CoraLite® Plus 488 ARL13B antibody (CL488-17711) at dilution of 1:200, CEP250/CNAP1 antibody (14498-1-AP, orange), SCLT1 antibody (14875-1-AP, Magenta).

  • Immunofluorescence (IF) / fluorescent staining of MDCK cells using CoraLite® Plus 488-conjugated ARL13B Polyclonal an (CL488-17711)

    IF Staining of MDCK using CL488-17711

    Immunofluorescent analysis of (4% PFA) fixed MDCK cells using CL488-17711 (ARL13B antibody) at dilution of 1:50 and CoraLite488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of hTERT-RPE1 cells using CoraLite® Plus 488-conjugated ARL13B Polyclonal an (CL488-17711)

    IF Staining of hTERT-RPE1 using CL488-17711

    Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using CoraLite® Plus 488 ARL13B antibody (CL488-17711) at dilution of 1:200, CoraLite®594 Acetyl-Tubulin (Lys40) antibody (CL594-66200, Clone: 7E5H8, red).

Tested Applications

Positive IF/ICC detected inhTERT-RPE1 cells, MDCK cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IFSee 2 publications below

Product Information

CL488-17711 targets ARL13B in IF/ICC applications and shows reactivity with human, mouse, rat, Canine samples.

Tested Reactivity human, mouse, rat, Canine
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ARL13B fusion protein Ag12015
Full Name ADP-ribosylation factor-like 13B
Calculated Molecular Weight 48 kDa
GenBank Accession NumberBC094725
Gene Symbol ARL13B
Gene ID (NCBI) 200894
RRIDAB_2883085
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ARL13B, also named as ARL2L1, is a small ciliary G protein of the Ras superfamily. Localized in the cilia, it is required for cilium biogenesis and sonic hedgehog signaling. Defects in ARL13B are the cause of Joubert syndrome (JS) which is an autosomal recessive disorder characterized by a distinctive cerebellar malformation (PMID: 19906870). CoraLite488-Conjugated ARL13B antibody can be used to mark the cilia(green). Ex: 488nm, Em: 515nm

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 ARL13B antibody CL488-17711Download protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Mol Oncol

Interferon regulatory factor 4 modulates epigenetic silencing and cancer-critical pathways in melanoma cells

Authors - Ulduz Sobhiafshar
View Article
mouseIF

Neurosci Res

Phenotypic characterization of Cdkl5-knockdown neurons establishes elongated cilia as a functional assay for CDKL5 Deficiency Disorder.

Authors - Alessia Di Nardo
View Article