HRP-conjugated IgA Monoclonal antibody

Human IgA Monoclonal Antibody for WB

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB

Conjugate

HRP

CloneNo.

3A5A5

Cat no : HRP-60099

Synonyms

FLJ14473, FLJ35065, FLJ35500, FLJ36402, FLJ39698, FLJ40001, FLJ41548, FLJ41552, FLJ41789, FLJ43248, FLJ43594, FLJ44293, FLJ46028, FLJ46621, FLJ46724, FLJ46811, FLJ46824, FLJ90170, Human IgA, IgA1, IGHA1

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Validation Data Gallery

• 

  WB analysis using HRP-60099

  50 ug, 25 ug, 12.5 ug human saliva were subjected to SDS PAGE followed by western blot with HRP-60099 (Human IgA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using HRP-60099

  human placenta tissue were subjected to SDS PAGE followed by western blot with HRP-60099 (Human IgA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human plasma using HRP-60099

  human plasma were subjected to SDS PAGE followed by western blot with HRP-60099 (Human IgA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: human saliva, human placenta tissue, human plasma

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

HRP-60099 targets Human IgA in WB applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Human IgA fusion protein Ag7417
• Full Name: immunoglobulin heavy constant alpha 1
• Calculated Molecular Weight: 496 aa, 53 kDa
• Observed Molecular Weight: 55-60 kDa
• GenBank Accession Number: BC016369
• Gene Symbol: IGHA1
• Gene ID (NCBI): 3493
• RRID: AB_2883830
• Conjugate: HRP
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Ig alpha is the major immunoglobulin class in body secretions. It may serve both to defend against local infection and to prevent access of foreign antigens to the general immunologic system. This antibody recognizes the Chain C region of human Ig alpha-1 (IGHA1).