CoraLite® Plus 647-conjugated IgA Monoclonal antibody

Human IgA Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IF

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

3A5A5

Cat no : CL647-60099

Synonyms

FLJ14473, FLJ35065, FLJ35500, FLJ36402, FLJ39698, FLJ40001, FLJ41548, FLJ41552, FLJ41789, FLJ43248, FLJ43594, FLJ44293, FLJ46028, FLJ46621, FLJ46724, FLJ46811, FLJ46824, FLJ90170, IgA1, IGHA1

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Validation Data Gallery

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  IF Staining of human tonsillitis using CL647-60099

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite® Plus 647 Human IgA antibody (CL647-60099, Clone: 3A5A5 ) at dilution of 1:400.

• 

  IF Staining of human tonsillitis using CL647-60099

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite® Plus 647 Human IgA antibody (CL647-60099, Clone: 3A5A5 ) at dilution of 1:400.

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Tested Applications

• Positive IF detected in: human tonsillitis tissue

Recommended dilution

• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL647-60099 targets Human IgA in IF applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Human IgA fusion protein Ag7417
• Full Name: immunoglobulin heavy constant alpha 1
• Calculated Molecular Weight: 496 aa, 53 kDa
• Observed Molecular Weight: 55-60 kDa
• GenBank Accession Number: BC016369
• Gene Symbol: Human IgA Heavy Chain
• Gene ID (NCBI): 3493
• RRID: AB_2920242
• Conjugate: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 654 nm / 674 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20^oC storage.

Background Information

Ig alpha is the major immunoglobulin class in body secretions. It may serve both to defend against local infection and to prevent access of foreign antigens to the general immunologic system. This antibody recognizes the Chain C region of human Ig alpha-1 (IGHA1).