IgA Monoclonal antibody

Human IgA Monoclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human and More (1)

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

Publications

2

CloneNo.

3A5A5

Cat no : 60099-1-Ig

Synonyms

Human IgA, IgA1, IGHA1

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Validation Data Gallery

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  WB analysis of human saliva using 60099-1-Ig

  human saliva were subjected to SDS PAGE followed by western blot with 60099-1-Ig (Human IgA Antibody) at dilution of 1:16000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human plasma using 60099-1-Ig

  human plasma were subjected to SDS PAGE followed by western blot with 60099-1-Ig (Human IgA Antibody) at dilution of 1:64000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human tonsillitis using 60099-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60099-1-Ig (Human IgA antibody) at dilution of 1:320000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 60099-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60099-1-Ig (Human IgA antibody) at dilution of 1:320000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human tonsillitis using 60099-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using 60099-1-Ig (Human IgA antibody) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: human saliva tissue, human saliva, human plasma
• Positive IHC detected in: human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: human tonsillitis tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:160000-1:640000
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 1 publications below

Product Information

60099-1-Ig targets Human IgA in WB, IHC, IF-P, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Human IgA fusion protein Ag7417
• Full Name: immunoglobulin heavy constant alpha 1
• Calculated Molecular Weight: 496 aa, 53 kDa
• Observed Molecular Weight: 55-60 kDa
• GenBank Accession Number: BC016369
• Gene Symbol: Human IgA Heavy Chain
• Gene ID (NCBI): 3493
• RRID: AB_1557448
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Caprylic acid/ammonium sulfate precipitation
• Storage Buffer: PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Ig alpha is the major immunoglobulin class in body secretions. It may serve both to defend against local infection and to prevent access of foreign antigens to the general immunologic system. This antibody recognizes the Chain C region of human Ig alpha-1 (IGHA1).

Publications

Species	Application	Title
		J Nanobiotechnology; A milk-based self-assemble rotavirus VP6-ferritin nanoparticle vaccine elicited protection against the viral infection.; Authors - Zhipeng Li
rat	IF	Eur J Pharmacol; Inhibition of excessive autophagy alleviates renal injury and inflammation in a rat model of immunoglobulin A nephropathy; Authors - Meiying Chang