Cardinal Red™-conjugated Histone H3 Polyclonal antibody
Histone H3 Polyclonal Antibody for FC (Intra)
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
FC (Intra)
Conjugate
Cardinal Red™ Fluorescent Dye
Cat no : CR-17168
Synonyms
Validation Data Gallery
Tested Applications
Positive FC detected in | HeLa cells |
Recommended dilution
Application | Dilution |
---|---|
Flow Cytometry (FC) | FC : 0.20 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
CR-17168 targets Histone H3 in FC (Intra) applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Histone H3 fusion protein Ag10644 |
Full Name | histone cluster 2, H3a |
Calculated Molecular Weight | 136 aa, 15 kDa |
Observed Molecular Weight | 15-17 kDa |
GenBank Accession Number | BC015544 |
Gene Symbol | Histone H3 |
Gene ID (NCBI) | 333932 |
Conjugate | Cardinal Red™ Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 592 nm / 611 nm |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Histone-H3, histone cluster 2, H3a is the core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machinery which requires DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Histone-H3 is expressed during S phase; then expression strongly decreases as cell division slows down during the process of differentiation.
Protocols
Product Specific Protocols | |
---|---|
FC protocol for Cardinal Red™ Histone H3 antibody CR-17168 | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |