Histone H3 Recombinant antibody, PBS Only

Histone H3 Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241079B2

Cat no : 81984-2-PBS

Synonyms

HIST2H3A, H3-clustered histone 14, H3-clustered histone 13, H3C15, H3C14

---

Validation Data Gallery

• 

  WB analysis using 81984-2-RR (same clone as 81984-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 81984-2-RR (Histone H3 antibody) at dilution of 1:25000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81984-2-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 81984-2-RR (same clone as 81984-2-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Histone H3 antibody (81984-2-RR, Clone: 241079B2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 81984-2-PBS in a different storage buffer formulation.

• 

  FC experiment of HepG2 using 81984-2-RR (same clone as 81984-2-PBS)

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug Histone H3 Recombinant antibody (81984-2-RR, Clone:241079B2) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 81984-2-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 81984-2-RR

  Biolayer interferometry (BLl) kinetic assays of 81984-2-RR against Human Histone H3 were performed. The affinity constant is 12.2 nM.

---

Product Information

The immunogen of 81984-2-PBS is Histone H3 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Histone H3 fusion protein Ag10644
• Full Name: histone cluster 2, H3a
• Calculated Molecular Weight: 136 aa, 15 kDa
• Observed Molecular Weight: 15 kDa
• GenBank Accession Number: BC015544
• Gene Symbol: Histone H3
• Gene ID (NCBI): 333932
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Histone-H3, histone cluster 2, H3a is the core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machinery which requires DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Histone-H3 is expressed during S phase; then expression strongly decreases as cell division slows down during the process of differentiation.