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  • Western Blot (WB) analysis of various lysates using Histone H3 Recombinant antibody (81984-2-RR)

    WB analysis using 81984-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 81984-2-RR (Histone H3 antibody) at dilution of 1:25000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Histone H3 Recombinant antibody (81984-2-RR)

    IF Staining of HeLa using 81984-2-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Histone H3 antibody (81984-2-RR, Clone: 241079B2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of HepG2 cells using Histone H3 Recombinant antibody (81984-2-RR)

    FC experiment of HepG2 using 81984-2-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug Histone H3 Recombinant antibody (81984-2-RR, Clone:241079B2) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • Affinity and Kinetic Characterization of 81984-2-RR

    Affinity and Kinetic Characterization of 81984-2-RR

    Biolayer interferometry (BLl) kinetic assays of 81984-2-RR against Human Histone H3 were performed. The affinity constant is 12.2 nM.

Histone H3 Recombinant antibody

Histone H3 Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 81984-2-RR
Clone No.241079B2

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, FC (Intra), ELISA

Synonyms

HIST2H3A, H3-clustered histone 14, H3-clustered histone 13, H3C15, H3C14

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

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Tested Applications

Positive WB detected inHeLa cells, A549 cells, mouse brain tissue, mouse kidney tissue, mouse skeletal muscle tissue, rat brain tissue
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

81984-2-RR targets Histone H3 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Histone H3 fusion protein Ag10644 Predict reactive species
Full Name histone cluster 2, H3a
Calculated Molecular Weight 136 aa, 15 kDa
Observed Molecular Weight15 kDa
GenBank Accession NumberBC015544
Gene Symbol Histone H3
Gene ID (NCBI) 333932
RRIDAB_3670510
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ71DI3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Histone-H3, histone cluster 2, H3a is the core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machinery which requires DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Histone-H3 is expressed during S phase; then expression strongly decreases as cell division slows down during the process of differentiation.

Protocols

Product Specific Protocols
WB protocol for Histone H3 antibody 81984-2-RRDownload protocol
IF protocol for Histone H3 antibody 81984-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols