Histone H3 Monoclonal antibody, PBS Only

Histone H3 Monoclonal Antibody for WB, IP, IF, FC, IHC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat, chicken, zebrafish, wheat

Applications

WB, IP, IF, FC, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1A2A3

Cat no : 68345-1-PBS

Synonyms

H3/A, H3FA, H3FB, H3FC, H3FD, H3FF, H3FH, H3FI, H3FJ, H3FK, H3FL, HIST1H3A, HIST1H3B, HIST1H3C, HIST1H3D, HIST1H3E, HIST1H3F, HIST1H3G, HIST1H3H, HIST1H3I, HIST1H3J, histone cluster 1, H3a, Histone H3, Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l, Histone-H3

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Validation Data Gallery

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  WB analysis using 68345-1-Ig (same clone as 68345-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68345-1-Ig (Histone H3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68345-1-PBS in a different storage buffer formulation.

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  WB analysis using 68345-1-Ig (same clone as 68345-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68345-1-Ig (HIST1H3A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68345-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 68345-1-Ig (same clone as 68345-1-PBS)

  IP result of anti-Histone H3 (IP:68345-1-Ig, 4ug; Detection:68345-1-Ig 1:2000) with HeLa cells lysate 1800 ug. This data was developed using the same antibody clone with 68345-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 68345-1-Ig (same clone as 68345-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 68345-1-Ig (HIST1H3A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68345-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 68345-1-Ig (same clone as 68345-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 68345-1-Ig (HIST1H3A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68345-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 68345-1-Ig (same clone as 68345-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Histone H3 antibody (68345-1-Ig, Clone: 1A2A3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 68345-1-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 68345-1-Ig (same clone as 68345-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using Histone H3 antibody (68345-1-Ig, Clone: 1A2A3 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 68345-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 68345-1-Ig (same clone as 68345-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Histone H3 antibody (68345-1-Ig, Clone: 1A2A3 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 68345-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 68345-1-Ig (same clone as 68345-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using Histone H3 antibody (68345-1-Ig, Clone: 1A2A3 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 68345-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 68345-1-Ig (same clone as 68345-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using Histone H3 antibody (68345-1-Ig, Clone: 1A2A3, labeled with CoraLite647-conjugated AffiniPure F(ab')2 Fragment Goat Anti-Mouse IgG (H+L),SA00014-10, magenta) at dilution of 1:400 and CoraLite Plus 488-conjugated ZO-1 antibody (CL488-21773, green), CHCHD6 antibody (66597-1-Ig, Clone: 2A11E9, labeled with FlexAble CoraLite Plus 555 Antibody Labeling Kit for Mouse IgG2a, KFA042, orange). This data was developed using the same antibody clone with 68345-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 68345-1-Ig (same clone as 68345-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Histone H3 antibody (68345-1-Ig, Clone: 1A2A3 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), Alpha Tubulin antibody (11224-1-AP, red). This data was developed using the same antibody clone with 68345-1-PBS in a different storage buffer formulation.

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  FC experiment of HepG2 using 68345-1-Ig (same clone as 68345-1-PBS)

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human Histone H3 (68345-1-Ig, Clone:1A2A3) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2b Isotype Control (MPC-11) (65128-1-Ig, Clone: MPC-11) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 68345-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68345-1-PBS targets Histone H3 in WB, IP, IF, FC, IHC, ELISA applications and shows reactivity with Human, mouse, rat, chicken, zebrafish, wheat samples.

• Tested Reactivity: Human, mouse, rat, chicken, zebrafish, wheat
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: histone cluster 1, H3a
• Observed Molecular Weight: 15 kDa
• GenBank Accession Number: BC066245
• Gene Symbol: HIST1H3A
• Gene ID (NCBI): 8350
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS only
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Histones are small, highly basic proteins that consist of a globular domain with unstructured N- and C-terminal tails protruding from the main structure. Histone H3 is one of the five main histones that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. In addition to their role in DNA compartmentalization, histones also play crucial roles in various biologic processes, including gene expression and regulation, DNA repair, chromatin condensation, cell cycle progression, chromosome segregation, and apoptosis. The ability of histones to regulate chromatin dynamics primarily originates from various posttranslational modifications carried out by histone-modifying enzymes.