Histone H3 Monoclonal antibody

Histone H3 Monoclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat, chicken, zebrafish, wheat, pig, rabbit, hamster, dog, canine and More (1)

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

CloneNo.

1A2A3

Cat no : 68345-1-Ig

Synonyms

H3/A, H3FA, H3FB, H3FC, H3FD, H3FF, H3FH, H3FI, H3FJ, H3FK, H3FL, HIST1H3A, HIST1H3B, HIST1H3C, HIST1H3D, HIST1H3E, HIST1H3F, HIST1H3G, HIST1H3H, HIST1H3I, HIST1H3J, histone cluster 1, H3a, Histone H3, Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l, Histone-H3



Tested Applications

Positive WB detected inLNCaP cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, MDCK cells, CHO cells, HeLa cells, chicken brain tissue, zebrafish, wheat whole plant
Positive IP detected inHeLa cells
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells, A431 cells, HeLa cells
Positive FC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:500-1:2000
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 9 publications below

Product Information

68345-1-Ig targets Histone H3 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with Human, mouse, rat, chicken, zebrafish, wheat, pig, rabbit, hamster, dog, canine samples.

Tested Reactivity Human, mouse, rat, chicken, zebrafish, wheat, pig, rabbit, hamster, dog, canine
Cited Reactivityhuman, mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Peptide
Full Name histone cluster 1, H3a
Observed molecular weight15 kDa
GenBank accession numberBC066245
Gene symbol HIST1H3A
Gene ID (NCBI) 8350
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Histones are small, highly basic proteins that consist of a globular domain with unstructured N- and C-terminal tails protruding from the main structure. Histone H3 is one of the five main histones that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. In addition to their role in DNA compartmentalization, histones also play crucial roles in various biologic processes, including gene expression and regulation, DNA repair, chromatin condensation, cell cycle progression, chromosome segregation, and apoptosis. The ability of histones to regulate chromatin dynamics primarily originates from various posttranslational modifications carried out by histone-modifying enzymes.

Protocols

Product Specific Protocols
WB protocol for Histone H3 antibody 68345-1-IgDownload protocol
IHC protocol for Histone H3 antibody 68345-1-IgDownload protocol
IF protocol for Histone H3 antibody 68345-1-IgDownload protocol
IP protocol for Histone H3 antibody 68345-1-IgDownload protocol
FC protocol for Histone H3 antibody 68345-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Redox Biol

Deficiency of S100 calcium binding protein A9 attenuates vascular dysfunction in aged mice

Authors - Boying Zhao
ratWB

Int J Mol Sci

Ursolic Acid Alleviates Neuroinflammation after Intracerebral Hemorrhage by Mediating Microglial Pyroptosis via the NF-κB/NLRP3/GSDMD Pathway

Authors - Pan Lei
humanWB

Curr Med Chem

Resveratrol Inhibits Hepatocellular Carcinoma Progression Through Regulating Exosome Secretion

Authors - Kun Tong
mouseWB

Brain Res

Neuronal pyroptosis mediated by STAT3 in early brain injury after subarachnoid hemorrhage

Authors - Shengjie Tang
humanWB

J Virol

HIV-1 Gag co-localizes with euchromatin histone marks at the nuclear periphery

Authors - Jordan Chang
humanWB

J Eur Acad Dermatol Venereol

Serine deficiency exacerbates psoriatic skin inflammation by regulating S-adenosyl methionine-dependent DNA methylation and NF-κB signalling activation in keratinocytes

Authors - Qinqin Meng