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CHCHD6 Monoclonal antibody

CHCHD6 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2A11E9

Cat no : 66597-1-Ig

Synonyms

CHCHD6

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CHCHD6 Monoclonal antibody (66597-1-Ig)

    WB analysis using 66597-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66597-1-Ig (CHCHD6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using CHCHD6 Monoclonal antibody (66597-1-Ig)

    IF Staining of MCF-7 using 66597-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CHCHD6 antibody (66597-1-Ig, Clone: 2A11E9 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

Tested Applications

Positive WB detected inHeLa cells, Jurkat cells, MCF-7 cells, HEK-293 cells
Positive IF/ICC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:3000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66597-1-Ig targets CHCHD6 in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen CHCHD6 fusion protein Ag14700
Full Name coiled-coil-helix-coiled-coil-helix domain containing 6
Calculated Molecular Weight 235 aa, 26 kDa
Observed Molecular Weight 26-29 kDa
GenBank Accession NumberBC006123
Gene Symbol CHCHD6
Gene ID (NCBI) 84303
RRIDAB_2881957
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for CHCHD6 antibody 66597-1-IgDownload protocol
IF protocol for CHCHD6 antibody 66597-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols