FITC-conjugated HBE1-Specific Monoclonal antibody

HBE1-Specific Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

FC (Intra)

Conjugate

FITC Fluorescent Dye

CloneNo.

2C11G6

Cat no : FITC-66151

Synonyms

HBE1, HBE1-Specific, Hemoglobin epsilon, Hemoglobin epsilon chain, Hemoglobin epsilon-Specific



Tested Applications

Positive FC detected inK-562 cells

Recommended dilution

ApplicationDilution
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of FITC-66151 is HBE1-Specific Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Fusion Protein
Full Name hemoglobin, epsilon 1
Observed Molecular Weight 16 kDa
GenBank Accession NumberNM_005330
Gene Symbol HBE1
Gene ID (NCBI) 3046
RRIDAB_2883828
Conjugate FITC Fluorescent Dye
Excitation/Emission Maxima Wavelengths494 nm / 520 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The hemoglobin molecule is a tetramer consisting of two α-globin-like polypeptide chains and two β-globin-like chains. The human hemoglobin genes are expressed in a tightly developmentally controlled fashion.ε-globin (HBE1) is the predominantly expressed gene during the embryonic stage. The epsilon hemoglobin chain seems to be the best marker for fetal nucleated red blood cells (NRBCs). Anti-HBE1 may be used to label and isolate fetal cells from maternal blood and can be useful in prenatal diagnosis. This antibody specifically recognizes the HBE1 and doesn't cross-react with other globin chains. This antibody is conjugated with FITC.

Protocols

Product Specific Protocols
FC protocol for FITC HBE1-Specific antibody FITC-66151Download protocol
Standard Protocols
Click here to view our Standard Protocols