• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

FITC-conjugated HBE1-Specific Monoclonal antibody

HBE1-Specific Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

FC (Intra)

Conjugate

FITC Fluorescent Dye

CloneNo.

2C11G6

Cat no : FITC-66151

Synonyms

Epsilon globin, HBE, HBE1, HBE1-Specific, Hemoglobin epsilon, Hemoglobin epsilon chain, Hemoglobin epsilon-Specific, Hemoglobin subunit epsilon, hemoglobin, epsilon 1

Validation Data Gallery

Filter:
  • Flow cytometry (FC) experiment of K-562 cells using FITC-conjugated HBE1-Specific Monoclonal antibody (FITC-66151)

    FC experiment of K-562 using FITC-66151

    1X10^6 K-562 cells were intracellularly stained with 0.4 ug FITC Anti-Human HBE1-Specific (FITC-66151, Clone:2C11G6) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive FC detected inK-562 cells

Recommended dilution

ApplicationDilution
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

FITC-66151 targets HBE1-Specific in FC (Intra) applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Recombinant protein
Full Name hemoglobin, epsilon 1
Observed Molecular Weight 16 kDa
GenBank Accession NumberNM_005330
Gene Symbol HBE1
Gene ID (NCBI) 3046
RRIDAB_2883828
Conjugate FITC Fluorescent Dye
Excitation/Emission Maxima Wavelengths498 nm / 526 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The hemoglobin molecule is a tetramer consisting of two α-globin-like polypeptide chains and two β-globin-like chains. The human hemoglobin genes are expressed in a tightly developmentally controlled fashion.ε-globin (HBE1) is the predominantly expressed gene during the embryonic stage. The epsilon hemoglobin chain seems to be the best marker for fetal nucleated red blood cells (NRBCs). Anti-HBE1 may be used to label and isolate fetal cells from maternal blood and can be useful in prenatal diagnosis. This antibody specifically recognizes the HBE1 and doesn't cross-react with other globin chains. This antibody is conjugated with FITC.

Protocols

Product Specific Protocols
FC protocol for FITC HBE1-Specific antibody FITC-66151Download protocol
Standard Protocols
Click here to view our Standard Protocols