CoraLite® Plus 647-conjugated HBE1-Specific Monoclonal antibody

HBE1-Specific Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

FC (Intra)

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

2C11G6

Cat no : CL647-66151

Synonyms

Epsilon globin, HBE, HBE1, HBE1-Specific, Hemoglobin epsilon, Hemoglobin epsilon chain, Hemoglobin epsilon-Specific, Hemoglobin subunit epsilon, hemoglobin, epsilon 1

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Validation Data Gallery

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  FC experiment of K-562 using CL647-66151

  1X10^6 K-562 cells were intracellularly stained with 0.2 ug CoraLite® Plus 647 Anti-Human HBE1-Specific (CL647-66151, Clone:2C11G6) (red), or 0.2 ug isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive FC detected in: K-562 cells

Recommended dilution

• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL647-66151 targets HBE1-Specific in FC (Intra) applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: hemoglobin, epsilon 1
• Observed Molecular Weight: 16 kDa
• GenBank Accession Number: NM_005330
• Gene Symbol: HBE1
• Gene ID (NCBI): 3046
• Conjugate: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 654 nm / 674 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

The hemoglobin molecule is a tetramer consisting of two α-globin-like polypeptide chains and two β-globin-like chains. The human hemoglobin genes are expressed in a tightly developmentally controlled fashion.ε-globin (HBE1) is the predominantly expressed gene during the embryonic stage. The epsilon hemoglobin chain seems to be the best marker for fetal nucleated red blood cells (NRBCs). Anti-HBE1 may be used to label and isolate fetal cells from maternal blood and can be useful in prenatal diagnosis. This antibody specifically recognizes the HBE1 and doesn't cross-react with other globin chains.