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CoraLite®594-conjugated HSPB3 Monoclonal antibody

HSPB3 Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG2b

Reactivity

Human, rabbit, mouse, rat

Applications

FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

2A10B5

Cat no : CL594-67890

Synonyms

HSPB3, HSPL27, Protein 3, 2A10B5, Heat shock protein beta-3, Heat shock protein family B member 3, HSP 17, HSP27

Validation Data Gallery

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  • Flow cytometry (FC) experiment of HeLa cells using CoraLite®594-conjugated HSPB3 Monoclonal antibody (CL594-67890)

    FC experiment of HeLa using CL594-67890

    1X10^6 HeLa cells were intracellularly stained with 0.2 ug CoraLite®594 Anti-Human HSPB3 (CL594-67890, Clone:2A10B5) (red), or 0.2 ug Mouse IgG2b Isotype Control (CL594-66360-3, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive FC (Intra) detected inHeLa cells
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-67890 targets HSPB3 in FC (Intra) applications and shows reactivity with Human, rabbit, mouse, rat samples.

Tested Reactivity Human, rabbit, mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen HSPB3 fusion protein Ag26940
Full Name heat shock 27kDa protein 3
Calculated Molecular Weight 150 aa, 17 kDa
Observed Molecular Weight20 kDa
GenBank Accession NumberBC126272
Gene Symbol HSPB3
Gene ID (NCBI) 8988
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Heat shock protein family B (small) member 3 (HSPB3) is the third member of the sHSP family in human and is mainly expressed in skeletal and smooth muscles, and can associate with HSPB2, which is another sHSP prominently expressed in cardiac and skeletal muscles. HSPB3 is involved in several cellular functions such as cell signaling, differentiation, and apoptosis, and mutations of HSPB3 are associated with neuromuscular diseases, such as axonal Charcot‐Marie‐Tooth disease (PMID: 31709619) (PMID: 32093037). The molecular weight of HSPB3 is about 17 kDa.

Protocols

Product Specific Protocols
FC protocol for CL594 HSPB3 antibody CL594-67890Download protocol
Standard Protocols
Click here to view our Standard Protocols