CoraLite® Plus 488-conjugated HSPB3 Monoclonal antibody

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  FC experiment of HeLa using CL488-67890

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human HSPB3 (CL488-67890, Clone:2A10B5) (red), or 0.4 ug Mouse IgG2b Isotype Control (CL488-66360-3, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

HSPB3 Monoclonal Antibody for FC (Intra)

Cat No. CL488-67890

Clone No.2A10B5

Host / Isotype

Mouse / IgG2b

Reactivity

Human, rabbit, mouse, rat

Applications

FC (Intra)

Synonyms

HSPB3, HSPL27, Protein 3, 2A10B5, Heat shock protein beta-3, Heat shock protein family B member 3, HSP 17, HSP27

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Tested Applications

• Positive FC (Intra) detected in: HeLa cells
• Positive FC detected in: HeLa cells

Recommended dilution

• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67890 targets HSPB3 in FC (Intra) applications and shows reactivity with Human, rabbit, mouse, rat samples.

• Tested Reactivity: Human, rabbit, mouse, rat
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: HSPB3 fusion protein Ag26940
• Full Name: heat shock 27kDa protein 3
• Calculated Molecular Weight: 150 aa, 17 kDa
• Observed Molecular Weight: 20 kDa
• GenBank Accession Number: BC126272
• Gene Symbol: HSPB3
• Gene ID (NCBI): 8988
• RRID: AB_2934582
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q12988
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Heat shock protein family B (small) member 3 (HSPB3) is the third member of the sHSP family in human and is mainly expressed in skeletal and smooth muscles, and can associate with HSPB2, which is another sHSP prominently expressed in cardiac and skeletal muscles. HSPB3 is involved in several cellular functions such as cell signaling, differentiation, and apoptosis, and mutations of HSPB3 are associated with neuromuscular diseases, such as axonal Charcot‐Marie‐Tooth disease (PMID: 31709619) (PMID: 32093037). The molecular weight of HSPB3 is about 17 kDa.