CoraLite® Plus 647-conjugated HIF-1 alpha Polyclonal antibody

• Featured Product
• KD/KO Validated

HIF-1 alpha Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 647 Fluorescent Dye

Cat no : CL647-20960

Synonyms

HIF1A, HIF 1α, HIF 1 alpha, Class E basic helix-loop-helix protein 78, Basic-helix-loop-helix-PAS protein MOP1

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Validation Data Gallery

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  IF Staining of HeLa using CL647-20960

  Immunofluorescent analysis of (-20°C Ethanol) fixed Cobalt Chloride treated HeLa cells using CoraLite® Plus 647 HIF-1 alpha antibody (CL647-20960) at dilution of 1:200.

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  FC experiment of HeLa using CL647-20960

  1x10^6 HeLa cells untreated (dashed lines) or treated with Cobalt Chloride (red) were intracellularly stained with 0.25 ug CoraLite® Plus 647-conjugated HIF-1 alpha Polyclonal antibody (CL647-20960), or 0.25 ug CoraLite® Plus 647-conjugated Rabbit IgG control Rabbit PolyAb (CL647-30000) (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

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Tested Applications

• Positive IF/ICC detected in: Cobalt Chloride treated HeLa cells
• Positive FC (Intra) detected in: Cobalt Chloride treated HeLa cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL647-20960 targets HIF-1 alpha in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HIF-1 alpha fusion protein Ag15198
• Full Name: hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor)
• Calculated Molecular Weight: 826 aa, 93 kDa
• Observed Molecular Weight: 120 kDa
• GenBank Accession Number: BC012527
• Gene Symbol: HIF1A
• Gene ID (NCBI): 3091
• Conjugate: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 654 nm / 674 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

HIF1a, the major regulator of the cellular responses to hypoxia, consists of an oxygen-sensitive subunit, HIF1 alpha (HIF1A), and an oxygen-insensitive subunit, HIF1 beta (arylhydrocarbon receptor nuclear transporter [ARNT]). Under normal oxygen conditions, HIF1a is continuously produced and destroyed, in a process involving hydroxylation, interaction with von Hippel-Lindau (VHL) protein, polyubiquitylation and subsequent proteasomal degradation. Under hypoxic conditions, hydroxylation is impaired and HIF1a is stabilized. HIF1a localizes in cytoplasm in normoxia, but it can translocate into nuclear in response to hypoxia. The calculated molecular weight of HIF1a is 93 kDa, but the modified protein HIF1a is about 110-120kDa (PMID: 11698256, .PMID: 7539918). .