CoraLite® Plus 488-conjugated HEY2 Recombinant antibody

• 

  IF Staining of HeLa using CL488-83501-7

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 HEY2 antibody (CL488-83501-7, Clone: 240474F2 ) at dilution of 1:200.

• 

  FC experiment of HeLa using CL488-83501-7

  1x10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488-conjugated HEY2 Recombinant antibody (CL488-83501-7, Clone:240474F2)(red), or 0.4 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

HEY2 Uni-rAb^TM Recombinant Antibody for IF/ICC, FC (Intra)

Cat No. CL488-83501-7

Clone No.240474F2

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra)

Synonyms

GRIDLOCK, Class B basic helix-loop-helix protein 32, CHF1, Cardiovascular helix-loop-helix factor 1, bHLHb32

---

Tested Applications

• Positive IF/ICC detected in: HeLa cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-83501-7 targets HEY2 in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: HEY2 fusion protein Ag30988
• Full Name: hairy/enhancer-of-split related with YRPW motif 2
• Calculated Molecular Weight: 36 kDa
• GenBank Accession Number: BC007707
• Gene Symbol: HEY2
• Gene ID (NCBI): 23493
• RRID: AB_3673261
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9UBP5
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Hairy/enhancer of split-related proteins, such as HEY2, are basic helix-loop-helix (bHLH) transcription factors implicated in cell fate decision and boundary formation. HEY genes are direct transcriptional targets of the Notch signaling pathways in Drosophila and vertebrates. HEY2 is similarly expressed in the somites whereas it shows a complementary expression in the heart, the craniofacial region and the nervous system. This antibody is a rabbit polyclonal antibody raised against full length of human HEY2.