HEY2 Recombinant antibody

HEY2 Uni-rAb^TM Recombinant Antibody for IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240474F2

Cat no : 83501-7-RR

Synonyms

GRIDLOCK, Class B basic helix-loop-helix protein 32, CHF1, Cardiovascular helix-loop-helix factor 1, bHLHb32

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Validation Data Gallery

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  IF Staining of HeLa using 83501-7-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HEY2 antibody (83501-7-RR, Clone: 240474F2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red).

• 

  IF Staining of HeLa using 83501-7-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HEY2 antibody (83501-7-RR, Clone: 240474F2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red).

• 

  FC experiment of HEK-293 using 83501-7-RR

  1x10^6 HEK-293 cells were intracellularly stained with 0.25 ug HEY2 Recombinant antibody (83501-7-RR, Clone:240474F2) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Control Antibody (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

• 

  FC experiment of HeLa using 83501-7-RR

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug HEY2 Recombinant antibody (83501-7-RR, Clone:240474F2) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

• 

  Affinity and Kinetic Characterization of 83501-7-RR

  Biolayer interferometry (BLl) kinetic assays of 83501-7-RR against Human HEY2 were performed. The affinity constant is 1.08 nM.

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Tested Applications

• Positive IF/ICC detected in: HeLa cells
• Positive FC (Intra) detected in: HeLa cells, HEK-293 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83501-7-RR is HEY2 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: HEY2 fusion protein Ag30988
• Full Name: hairy/enhancer-of-split related with YRPW motif 2
• Calculated Molecular Weight: 36 kDa
• GenBank Accession Number: BC007707
• Gene Symbol: HEY2
• Gene ID (NCBI): 23493
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Hairy/enhancer of split-related proteins, such as HEY2, are basic helix-loop-helix (bHLH) transcription factors implicated in cell fate decision and boundary formation. HEY genes are direct transcriptional targets of the Notch signaling pathways in Drosophila and vertebrates. HEY2 is similarly expressed in the somites whereas it shows a complementary expression in the heart, the craniofacial region and the nervous system. This antibody is a rabbit polyclonal antibody raised against full length of human HEY2.